Effect of incorporation of cobalt ions into hydroxyapatite and its release on osteoclast differentiation and resorptive activity

ผลของการผสมโคบอลต์ไอออนเข้าไปในไฮดรอกซี่อะพาไทต์และการปลดปล่อยของโคบอลต์ไอออนจากไฮดรอกซี่อะพาไทต์ที่มีผลต่อการแบ่งตัวและความสามารถในการละลายไฮดรอกซี่อะพาไทต์ของออสทีโอคลาส

Name: Tuangkun Mukkayadachochai

keyword: Hydroxyapatite

; Cobalt ions Ion exchange

; Osteoclasts

; Osteoclast differentiation

; Resorptive area

Abstract: Synthetic hydroxyapatite (HA) is developed to enable minimal invasive surgery and to address high demands for bone augmentation and regeneration in dental implant patients. Since HA has low solubility rate, a concept behind the study was to incorporate metal ions into HA to improve limitation of hydroxyapatite solubility property. Cobalt ion was selected because it can stimulate osteoclastogenesis and resorptive activity by mimicking hypoxia pathway. In this study, different concentrations of cobalt ions (Co2+) 40, 400, 4000 and 8000 μM were incorporated into HA via ion exchange method. The resulting samples were uniaxially pressed into the disc form and sintered at temperature at 1100°C. Microstructure, chemical, and physical properties of the HA and cobalt ion-incorporated HA (HACo) were characterized. Subsequently, RAW 264.7 cells were cultured in the presence of RANKL on these discs to examine the effects of Co2+ on osteoclast formation and resorptive activity. In viability test, there was no statistically significant difference from control group of the cells in all disc conditions at day 7. The release of cobalt ions in the culture media was related to the concentration of Co2+ doping. It was found that Co2+ increased the area of TRAP staining. In general, the Co2+ concentrations of 40-400 μM were effective in increasing TRAP staining area. HACo 400 μM significantly increased the area by 4-fold of control, and followed by HACo 40 μM which stimulated osteoclast differentiation by 3.2-fold. In addition, TRAP activity and number of nuclei showed no statistically significant differences between all groups. HACo 40-4000 μM demonstrated statistically significant increase in resorption area when compared with control. The most effective was observed at HACo 400 μM by 6-fold of control. In conclusion, this study showed the potential of this relatively simple approach to induce osteoclast resorption of HA. The 40-400 μM of Co2+ were favorable concentrations to improve limitations of HA as bone substituted biomaterials

Thammasat University. Thammasat University Library

Address: BANGKOK

Email: preserv@tu.ac.th

Name: Somying Pattirapong

Role: advisor

Created: 2021

Modified: 2023-11-20

Issued: 2023-11-20

วิทยานิพนธ์/Thesis

application/pdf

DOI: 10.14457/TU.the.2021.1192

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Dentistry

Grantor: Thammasat University

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