Molecular diversity of poleroviruses inflecting cucurbits in Thailand

Name: Sirirat Cheewachaiwit

LCSH: Kasetsart University -- Theses. M.S. (Agricultural Biotechnology) 2017

Classification :.LCCS: SB608.C885

LCSH: Kasetsart University. Interdisciplinary Graduate Program -- Theses

LCSH: Cucurbitaceae -- Diseases and pests

LCSH: Luteoviridae

LCSH: Plant viruses

Abstract: Field surveys and sample collection were conducted during February 2012 - August 2013. A total of 244 cucurbit leaves with yellowing symptom and weeds were collected from 15 provinces in Thailand. Initial screening by RT-PCR using degenerate primers Luteo-CPF and Luteo-CPR indicated that 160 samples (~66%) from bottle gourd, bitter gourd, cucumber, luffa, melon, pumpkin wax gourd and weeds were infected by polerovirus. Of the160 positive samples, 27 samples from different cucurbit crops collected from different locations were selected to amplify a 1.4 kb region covering partial RdRp gene, IR and full CP gene using primers PoconF and PococpR. The amplified PCR products were cloned and sequenced. The results indicated that 4 distinct species of polerovirus were found in relation to yellowing disease in cucurbit plants and they were identified to be Cucurbit aphid borne yellows virus (CABYV), Luffa aphid-borne yellows virus (LABYV), Melon aphid-borne yellows virus (MABYV) and Suakwa aphid-borne yellows virus (SABYV). In addition, the recombinant strain of CABYV (CABYV-R) and SABYV (SABYV-R) was identified. CABYV-R was similar to the one reported in Taiwan, Philippines and Thailand. But this is the first report of SABYV-R found in Thailand. From further analysis by SIMPLOT, BOOTSCAN and RDP programs, CABYV-R derived from MABYV and CABYV-C with CABYV-C identified as a major parent. Whereas SABYV-R derived from MABYV and SABYV with SABYV identified as a major parent.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Chatchawankanphanich

Role: Thesis Advisor

Name: Ratchanee Hongprayoon

Role: Thesis Co-Advisor

Created: 2017

Modified: 2023-06-29

Issued: 2023-06-29

วิทยานิพนธ์/Thesis

application/pdf

URL: ttps://kasetsart.idm.oclc.org/login?url=https://www.lib.ku.ac.th/KUthesis/2560/sirirat-che-all.pdf

CallNumber: SB608.C885.S57

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Agricultural Biotechnology

Grantor: Kasetsart University

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