Hydrogen energy from rubrivivax gelatinosus SB24 : efficiency in hydrogen production upon the bacterial physiology constraint and microbial technology

Name: Wilawan Channarong

LCSH: Kasetsart University -- Dissertations. Ph.D. (Microbiology) 2017

Classification :.LCCS: QD181.H4

LCSH: Kasetsart University. Department of Microbiology -- Dissertations

LCSH: Hydrogen

LCSH: Rubrivivax

LCSH: Bacteria

Abstract: Pure hydrogen was successfully produced by Rubrivivax gelatinosus SB24 using a patented photobioreactor (patent NO.49222), with various starch. BioH2 obtained from starch were 7-13 L by 5.7 L culture and 74-99% of starch was utilized. A methane production process was connected to renewable utilization of suspended solution remained. COD was 96% reduced with the benefit of gaining CH4, 4 L/4 days. The later system could be maintained for 14 months. The process for the alternative energy production was a zerowaste operation. Improvement of the H2 production process was successful with either using semicontinuous culture or cell immobilization. Repeated batch with 25 and 50% withdrawn and repeated fed batch with 10x diluted N-source could improve the process 2-3 times. Cell immobilization, with 48 h cell entrapped in 3% agar was the best technic and improved the H2 process 9 times. The process continued for 71 days. Mathematical modeling using Box-Behnken Design and MINITAB/STATISTICA showed how the 4 factors and their interactions affected cell efficiency in H2 production and growth. There was interaction effect of light intensity with C-conc. and that with pH affected on H2 metabolism while light intensity and N-conc. had interaction effect on growth. Optimal conditions for cell efficiency in H2 production was C:N ratio 90:1, pH 5.8 and 10 klux light intensity and those for growth was 18:1, 5.8 and 1 klux, respectively. This is the first informative reports showing the 4 factors affecting cell efficiency in H2 production and growth differently. It showed that cell efficiency in H2 production could be maximized with low N-conc. of C:N ratio 90:1 and extremely high light intensity, 10 klux. In contrast, cell efficiency in growth needed higher N-conc. of C:N ratio 18:1 under low light intensity, 1 klux. It was at the first time, reported the interaction between the factors that affected the 2 metabolisms in different manners.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Lerluck Chitradon

Role: Thesis Advisor

Created: 2017

Modified: 2023-06-28

Issued: 2023-06-28

วิทยานิพนธ์/Thesis

application/pdf

URL: https://kasetsart.idm.oclc.org/login?url=https://www.lib.ku.ac.th/KUthesis/2560/wilawan-cha-all.pdf

CallNumber: QD181.H4.W54

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Microbiology

Grantor: Kasetsart University

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