Immobilization of lipase by surfactant CTAB-modified bentonite and namoclay for the production of cosmetic esters

Name: Salisa Wonethepbut

Organization : Chiang Mai University. Faculty of Graduate School

Name: Kanchana Dumri

Organization : Chiang Mai University. Faculty of Science

Email : kanchana.d@cmu.ac.th

keyword: Cetyltrimethylammonium bromide

LCSH: Bentonite

; Enzyme immobilization

LCSH: Surfactants

Abstract: Natural clay and nanoclay have gained enormous interest in the enzyme immobilization field due to their improving activity and efficiency stability of the enzyme. The main objective of this study was to produce the cosmetic esters through immobilized lipase of Candida rugosa on supporting materials e.g.. bentonite. modified bentonite, and nanoclay. Bentonite was successfully modified using the cationic surfactant cetyltrimethylammonium bromide (CTAB). The supporting materials and immobilized lipase were characterized by a Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectrometer (FTIR). The catalytic activities of lipase were carried out under optimal conditions of pH 7.0 and 40 으C. Free lipase (FL), immobilized lipase on bentonite (IL on B), and immobilized lipase on modified bentonite (IL on MB) were 5.73 士 0.37, 0.85 - 0.86, and 3.96 t 0.08 U/g, respectively. The modified bentonite could be improved lipase activity over unmodified bentonite, even though free lipase displayed the highest activity. However, this research will be further focused on other factors e.g.. Oolipase immobilization on supporting material, stability at different pH and temperature as well as the reusability of immobilized enzymes. In addition, immobilized lipase on selected support will be prepared as a biocatalyst to contribute the cosmetic ester production.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2023

Modified: 2023-06-26

Issued: 2023-06-26

บทความ/Article

application/pdf

BibliograpyCitation : In Mae Fah Luang University. School of Science. The Pure and Applied Chemistry International Conference 2023 (PACCON 2023) (pp.717-721). Chiang Rai : Mae Fah Luang University

eng

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