Characterization of organellar genomes in oil palm (Elaeis guineensis Jacq.)

Name: Pichahpuk Uthaipaisanwong

LCSH: Kasetsart University -- Dissertations. Ph.D. (Genetic Engineering) 2017

Classification :.LCCS: QK495.P17

LCSH: Kasetsart University. Interdisciplinary Graduate Program -- Dissertations

LCSH: Oil palm -- Genetics

LCSH: Palm oil

LCSH: Genetic markers

LCSH: Genomes

Abstract: Plant cells contain three genomes including nuclear genome and two other organellar genomes, which are chloroplast (cp) and mitochondrial (mt) genomes. There are many studies about the use of nuclear genome of oil palm (Elaeis guineensis Jacq.) but only few about organellar genomes due to the lack of genome sequence information. The advancement of the high throughput sequencing and computational technologies allow us to perform genome sequencing and analyzing the genome sequence. In this study, (1) the completed oil palm chloroplast genome sequence was obtained using 454 pyrosequencing. The genome contains 113 unique genes, which are well conserved with previously characterized cp genomes of plant species in Poales group. Fifty common protein-coding genes were used to construct phylogenetic trees for 34 plant taxa to investigate the genetic relationships. The phylogenetic analysis provided a congruent tree with previous studies and also the placement of oil palm as the sister of closely related date palm in family Arecaceae. (2) The oil palm mt sequences (258 contigs) were assembled from reads that aligned to the homologous regions of date palm mt genome (covered ~57%). Ten mt simple sequence repeat (mtSSR) markers were developed from the candidate mt sequences of E. guineensis to study the genetic diversity among fifteen plant species distributed in different subfamilies in Arecaceae and thirteen genetically diverse E. guineensis accessions. Nine of ten markers, developed from oil palm mt genome sequence, were transferable and polymorphic in 15 Arecaceae species, while ten mtSSR and thirteen mtSNP markers did not show polymorphism among 13 E. guineensis accessions. In conclusion, we report the completed sequence of oil palm cp genome and the mtSSR, developed from oil palm mt sequences, could be a resource for the genetic diversity study in Arecaceae species.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Chatchawan Jantasuriyarat

Role: Thesis Advisor

Name: Sithichoke Tangphatsornruang

Role: Thesis Co-Advisor

Name: Suthasinee Somyong

Role: Thesis Co-Advisor

Created: 2017

Modified: 2023-06-23

Issued: 2023-06-23

วิทยานิพนธ์/Thesis

application/pdf

URL: https://kasetsart.idm.oclc.org/login?url=https://www.lib.ku.ac.th/KUthesis/2560/pichahpuk-uth-all.pdf

CallNumber: QK495.P17.P53

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Genetic Engineering

Grantor: Kasetsart University

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