Nwai Oo Khine. Detection of giardia duodenalis and cryptosporidium spp. in stray dogs of Bangkok areas, Thailand. Master's Degree(Bio-Veterinary Science). Kasetsart University. Office of the University Library. : Kasetsart University, 2017.
Detection of giardia duodenalis and cryptosporidium spp. in stray dogs of Bangkok areas, Thailand
Abstract:
Both Cryptosporidium spp. and Giardia duodenalis are enteric protozoan parasites that infect a wide variety of domestic animals as well as humans worldwide causing diarrheal diseases. Giardia duodenalis assemblages C and D are specific to canine hosts and zoonotic assemblages A and B are also found in dogs as a reservoir host. In dogs, Cryptosporidium canis is the host specific species and humans are infected by C. hominis and C. parvum. The objective of this study was to determine the prevalence of Cryptosporidium spp. and Giardia duodenalis from stray dogs of Bangkok areas and to identify the species and assemblages. In 2015, 540 dog fecal samples were collected from 95 monasteries of 48 districts from June to December. For microscopic examination, DMSO-modified acid fast staining was used for Cryptosporidium spp. oocysts and zinc-sulfate fecal examination for G. duodenalis. The nested PCR was performed using 18S rRNA gene for both parasites. A total of 2.96% (16/540) samples were found positive for G. duodenalis by PCR method, 0.74% (4/540) from zinc-sulfate fecal examination. The majority was G. duodenalis assemblage D followed by assemblage C and zoonotic assemblages B and A. The prevalence of Cryptosporidium spp. was 0.74% (4/540) by PCR method and all were dog genotypes C. canis. These results indicated that dogs resided in Bangkok monasteries might be the major risk of public health concern since they were sharing the environment with humans.
Kasetsart University. Office of the University Library