Physico-chemical changes and gene expression involved in carotenoid biosynthesis of durian pulp during growth and ripening

Name: Apinya Wisutiamonkul

LCSH: Kasetsart University -- Dissertations. Ph.D. (Horticulture) 2017

Classification :.LCCS: SB379.D8

LCSH: Kasetsart University. Department of Horticulture -- Dissertations

LCSH: Durian -- Genetic aspects

LCSH: Durian -- Postharvest technology

LCSH: Carotenoids

LCSH: Gene expression

LCSH: Biosynthesis

Abstract: Carotenoid content in durian (Durio zibethinus) fruit is an important aspect of fruit quality. Total carotenoid content was approximately 6-fold higher in ‘Chanee’ than in ‘Monthong’. The major carotenoids in the fruit pulp were beta carotene and alpha-carotene, with minor carotenoids being lutein and zeaxanthin. Significant differences in beta-carotene and alpha-carotene levels were seen between the two cultivars. Zeta-carotene desaturase (ZDS) expression positively correlated with increases in total carotenoids, beta-carotene, alpha-carotene, and lutein content and lycopene epsilon-cyclase (LCYE) expression correlated with total carotenoids and beta-carotene content. In contrast, lycopene beta-cyclase (LCYB) showed a negative correlation with total carotenoids, beta-carotene, alpha-carotene and lutein. It can be concluded that carotenoid accumulation during fruit growth is regulated by the balanced expression of the ZDS, LCYE and LCYB. Fruit of ‘Chanee’ harvested at the mature stage were either untreated, treated with ethephon, 1-methylcyclopropene (1-MCP), or 1-MCP followed by ethephon, then stored at 25 oC. 1-MCP delayed the increase in beta-carotene, alpha-carotene, and zeaxanthin, but not lutein. In contrast, ethephon had no significant effect on carotenoid level. The expression of ZDS, LCYB, chromoplast specific lycopene beta-cyclase (CYCB) and beta-carotene hydroxylase (BCH) was highly correlated with carotenoid content and pulp color. 1-MCP resulted in significant down-regulation of ZDS, LCYB, CYCB and BCH expression. The functional test of LCYB in bacteria showed conversion of lycopene and delta-carotene to beta-carotene and alpha-carotene, respectively. These results suggest that ripening-induced carotenoid accumulation is regulated by endogenous ethylene controlling the expression of key genes such as LCYB.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Emeritus Saichol Ketsa

Role: Thesis Advisor

Name: Wachiraya Imsabai

Role: Thesis Co-Advisor

Name: Parichart Burns

Role: Thesis Co-Advisor

Created: 2017

Modified: 2023-06-14

Issued: 2023-06-14

วิทยานิพนธ์/Thesis

application/pdf

URL: https://kasetsart.idm.oclc.org/login?url=https://www.lib.ku.ac.th/KUthesis/2560/apinya-wis-all.pdf

CallNumber: SB379.D8.A65

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Horticulture

Grantor: Kasetsart University

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