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Tanakorn Srirat. Sequence variation in rice blast resistance gene, Piz-t and rice blast fungus effector gene, AvrPiz-t and efficiency of using japonica rice variety, toride 1 as a novel source of blast resistance in Thailand. Master's Degree(Genetics). Kasetsart University. Office of the University Library. : Kasetsart University, 2016.
Sequence variation in rice blast resistance gene, Piz-t and rice blast fungus effector gene, AvrPiz-t and efficiency of using japonica rice variety, toride 1 as a novel source of blast resistance in Thailand
Name: Tanakorn Srirat
Classification :.LCCS: QK495.G74
LCSH:
Rice
-- Disease and pest resistance
LCSH:
DNA
LCSH:
Genetic markers
Abstract:
Rice (Oryza sativa L.) is a staple food for over one third of the world's population, including the inhabitants of Thailand. The production and rate of productivity of rice is constrained by factors that impact the yield of rice worldwide. Sixteen diseases have a major degree of economic influence on rice production, but rice blast disease is the most important disease in rice-growing countries worldwide. Rice blast, caused by Magnaporthe oryzae, is recognized as the most significant and potentially the most damaging disease, which afflicts rice in temperate and tropical regions. Therefore, breeding blastresistance cultivars is a desirable mean of controlling rice blast. Toride1, Japonica rice has a potential to combat blast disease because it carries many genes involved in blast disease, including Piz-t. Therefore, this study aims to develop gene specific functional marker for investigate rice blast resistance gene, Pizt in landrace Thai rice and to examine the nucleotide sequence variation of avirulence gene, AvrPiz-t that influences the adaptation of rice blast fungus to overcome the resistance. Lastly, the disease spectrum of Toride1 against Thailand rice blast isolates was revealed. The results showed that 25% of landrace Thai rice have Piz-t resistance allele. Sequence variation of AvrPiz-t in Thai rice blast isolates were revealed 57 nucleotide polymorphic sites resulted in 3 different haplotypes. Haplotype diversity index (0.151) and nucleotide diversity index (0.01740) showed low level of genotypic diversity within Thai rice blast isolates. Finally, Toride1 showed a broad spectrum phenotype to Thai rice blast isolates (91.30%). In conclusion, the newly marker that was developed from this study will be beneficial in tracking Piz-t for blast resistance in rice breeding program and the information about genetic diversity of Thai rice blast fungus obtained from this study will be crucial for understanding the diversity of pathogenic population and pathogenic selection, which can lead to the novel strategic development for co-evolutionary relationship between Rand Avr genes in rice breeding programs.
Kasetsart University. Office of the University Library
Address:
Bangkok
Email:
tdckulib@ku.ac.th
Role:
Thesis Advisor
Name: Pattavipha Songkumarn
Role:
Thesis Co-Advisor
Created:
2016
Modified:
2023-06-12
Issued:
2023-06-12
วิทยานิพนธ์/Thesis
application/pdf
URL: https://kasetsart.idm.oclc.org/login?url=https://www.lib.ku.ac.th/KUthesis/2559/tanakorn-sri-all.pdf
CallNumber:
QK495.G74.T36
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Genetics
Grantor: Kasetsart University
©copyrights Kasetsart University
RightsAccess:
ใช้เวลา
0.027154 วินาที
Tanakorn Srirat
| Title | Contributor | Type |
|---|---|---|
| Sequence variation in rice blast resistance gene, Piz-t and rice blast fungus effector gene, AvrPiz-t and efficiency of using japonica rice variety, toride 1 as a novel source of blast resistance in Thailand
มหาวิทยาลัยเกษตรศาสตร์ Tanakorn Srirat | Chatchawan Jantasuriyarat Pattavipha Songkumarn | วิทยานิพนธ์/Thesis |
Chatchawan Jantasuriyarat
Pattavipha Songkumarn