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Ratchaneewan Audpad. Antioxidant activity of protein from Psophocarpus tetragonolobus seeds enhanced by gamma radiation. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2021.
Antioxidant activity of protein from Psophocarpus tetragonolobus seeds enhanced by gamma radiation
Name: Mathira Wongchai
Organization :
Thammasat University. Faculty of Allied Health Sciences
Name: Anirut Hlosrichok
Organization :
Thammasat University. Faculty of Allied Health Sciences
Name: Ratchaneewan Audpad
Organization :
Thammasat University. Faculty of Allied Health Sciences, Thammasat University Research Unit in Antimicrobial Agents and Application
Email :
aratchan@tu.ac.th
keyword:
Psophocarpus tetragonolobus
ThaSH:
Winged bean
ThaSH:
Antioxidants
ThaSH:
Gamma rays
ThaSH:
Plant proteins
Abstract:
The objective of this research is to apply gamma radiation or electron beam to promote
the protein extraction from Psophocarpus tetragonolobus seeds. The yield and antioxidant
activity of proteins extracted from Psophocarpus tetragonolobus seeds irradiated with gamma
or electron beam and non-irradiated seed using acid-based solution methods was compared.
Extraction of proteins from gamma-irridated seeds showed the highest percent yield (4.95 ± 0.74 percent)
when compared to that of electron beam-irridated (4.65 ± 0.56 percent) and non-irridated seeds (3.23
± 0.64 percent). Moreover, the proteins extracted from gamma-irridated seeds exhibited the highest
antioxidant activity with IC50 value of 45.38 ± 6.48 Mu g/ml and 47.08 ± 1.75 Mu g/ml as
determined by DPPH and ABTS assay, respectively. The major proteins extracted from
Psophocarpus tetragonolobus seeds had the molecular weight ranging from 15-20 kDa and 75-
245 kDa as shown in SDS-PAGE. In conclusion, the gamma radiation could enhance both the
amount and the antioxidant activity of the protein extracted from Psophocarpus tetragonolobus
seeds.
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2021
Modified:
2023-06-09
Issued:
2023-06-09
บทความ/Article
application/pdf
BibliograpyCitation :
In Thai Society for Biotechnology. Thai Society for Biotechnology and International Conference Online (TSB Online 2021) (pp.634-640). Bangkok : Thai Society for Biotechnology
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.034249 วินาที
Mathira Wongchai
| Title | Contributor | Type |
|---|---|---|
| Antioxidant activity of protein from Psophocarpus tetragonolobus seeds enhanced by gamma radiation
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Mathira Wongchai;Anirut Hlosrichok;Ratchaneewan Audpad | บทความ/Article |
Anirut Hlosrichok
| Title | Contributor | Type |
|---|---|---|
| Antioxidant activity of protein from Psophocarpus tetragonolobus seeds enhanced by gamma radiation
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Mathira Wongchai;Anirut Hlosrichok;Ratchaneewan Audpad | บทความ/Article |
Ratchaneewan Audpad
| Title | Contributor | Type |
|---|---|---|
| Antioxidant activity of protein from Psophocarpus tetragonolobus seeds enhanced by gamma radiation
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Mathira Wongchai;Anirut Hlosrichok;Ratchaneewan Audpad | บทความ/Article |