Effects of season and preservation on gamete quality in rusa deer (Rusa timorensis)

Name: Apichaya Sudsukh

LCSH: Kasetsart University -- Dissertations. Ph.D. (Agricultural Biotechnology) 2016

Classification :.LCCS: SF401.D3

LCSH: Kasetsart University. Interdisciplinary Graduate Program -- Dissertations

LCSH: Deer -- Physiology

LCSH: Cervus timorensis

Abstract: This study was divided into 4 parts according to male and female reproductive physiology of rusa deer. The objective of the first part was to determine the relationship between testicular and epididymal histological structure and cellular source of testicular inhibin in rusa deer at different antlers status. Eighteen rusa deer testes and epididymides were collected. They were separated into three groups depending on antlers stages. The histological structures and morphology of cells were stained with haematoxylin-eosin (H&E). Means (±SEM) of testicular weight and seminiferous tubulars diameter in hard antler group (76.25±9.80 g and 256.10 ± 7.28 µm) was significantly higher than velvet antlered stags (40.86±7.41 g and 197.25 ± 7.23 µm) (p<0.05). In the hard antler groups, their epididymides were filled up widely with numerous spermatozoa. Hard antlered rusa deer semen was collected. Then, frozen-thawed semen was incubated in different media for capacitatation and hyperactivation assessments. The objective of the second part of the study was to determine the effect of capacitation media in vitro on frozen-thawed rusa deer spermatozoa by computer assisted sperm analyzer (CASA). The suitable capacitation media supplement was 0.6% (w/v) bovine serum albumin (BSA) for activating rusa deer spermatozoa. The objective of the third part was to evaluate fecal progesterone and 17βestradiol metabolite profiles in rusa hinds (n=5) throughout the year. Rusa deer fecal samples were collected weekly and subjected to hormone extraction and subsequently hormone metabolite analysis using enzyme- and radio- immunoassay. The results indicated that they appeared to have reproductive cycle throughout the year. The objective of the forth part was to examine the effects of breeding season on the ovarian structure, quality in vitro and maturation of rusa deer oocytes. Ninety-four ovaries were recovered and ovarian structures and oocyte quality were evaluated. The immature oocytes were matured in vitro for 24 hours in order to assess the maturation success. Breeding season had significant effect only on ovary structure not for in vitro meiotic competence. In conclusion, the female rusa deer is not clearly a seasonal breeder whereas male rusa deer is strongly seasonal breeder in Thailand. These findings provide important information about the rusa deer reproduction which can be used as elemental data for improving the success of assisted reproductive technologies (ARTs) in the future.

Kasetsart University. Office of the University Library

Address: Bangkok

Email: tdckulib@ku.ac.th

Name: Nikorn Thongtip

Role: Thesis Advisor

Name: Ampika Thongphakdee

Role: Thesis Co-Advisor

Name: Theerawat Tharasanit

Role: Thesis Co-Advisor

Created: 2016

Modified: 2023-06-07

Issued: 2023-06-07

วิทยานิพนธ์/Thesis

application/pdf

URL: https://kasetsart.idm.oclc.org/login?url=https://www.lib.ku.ac.th/KUthesis/2559/apichaya-sud-all.pdf

CallNumber: SF401.D3.A64

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Agricultural Biotechnology

Grantor: Kasetsart University

©copyrights Kasetsart University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	apichaya-sud-all.pdf	6.48 MB

ใช้เวลา

0.035654 วินาที