Infection density, diversity and distribution of Wolbachia Bacteria in Moths (order Lepidoptera): first systematic report from Thailand

Name: Panukorn Boonsit

Organization : King Mongkut's Institute of Technology Ladkrabang. Center of Excellence in Applied Bioscience, King Mongkut's Institute of Technology Ladkrabang. Faculty of Agricultural Technology

Name: Itsanun Wiwatanaratanabutr

Organization : King Mongkut's Institute of Technology Ladkrabang. Center of Excellence in Applied Bioscience, King Mongkut's Institute of Technology Ladkrabang. Faculty of Agricultural Technology

Email : tsanun.wi@kmitl.ac.th

keyword: Wolbachia Bacteria

ThaSH: Moths -- Infection

ThaSH: Lepidoptera

ThaSH: Gram-negative bacterial infections

Abstract: Members of the genus Wolbachia are a group of Rickettsia-like, intracellular, maternally inherited bacterial endosymbionts that infect a diverse range of insects and cause reproductive changes in their hosts. Although Wolbachia's role in many insects has been extensively studied, a little is known about Wolbachia in Lepidopteran which is one of the most diverse insects. Here, we present the first survey of the Wolbachia infection status in different species of moths from three different geographic regions of Thailand, which was carried out during January to December in 2019 with the screening of 1,235 specimens in total of 58 moth species from 13 families. Specimens were collected from Khao Yai National Park (the Central and the Northeast regions), and Kaeng Krachan National Park (the West region). Infections of Wolbachia were screened by using polymerase chain reaction with 16S rRNA, ftsZ and wsp gene primers which the results indicated high rates of Wolbachia infection in moth populations from Thailand. Wolbachia was found in all different geographically populations in total of 625 individuals in total of 28 moth species from 9 families, including 144 individuals (46 males and 98 females) from the Central, 156 individuals (49 males and 107 females) from the Northeast, and 325 individuals (114 males and 211 females) from the West. The highest infection rate was 90.47 percent in the West populations and the average infection rate was 61.90 percent. The detection of Wolbachia in different moth populations from all regions was identical when all primers were used to screen for Wolbachia. A bit difference of detection rate in PCR assay was not a significant difference. The relative densities of Wolbachia within each individual were determined using quantitative real-time PCR and the result showed that there was a low Wolbachia infection density in these moth populations. These findings indicated that Wolbachia are distributed throughout the moth populations from Thailand.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2021

Modified: 2023-05-08

Issued: 2023-05-08

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BibliograpyCitation : In Thai Society for Biotechnology. Thai Society for Biotechnology and International Conference Online (TSB Online 2021) (pp.121-133). Bangkok : Thai Society for Biotechnology

eng

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