Administration of the Recombinant WSSV-rVP28 activates shrimp phagocytic activity

Name: Naing Win Htut

Organization : Mahidol University. Faculty of Science

Name: Suparat Taengchaiyaphum

Organization : National Science and Technology Development Agency (NSTDA). National Center for Genetic Engineering and Biotechnology (BIOTEC)

Name: Kallaya Sritunyalucksana

Organization : Mahidol University. Faculty of Science, National Science and Technology Development Agency (NSTDA). National Center for Genetic Engineering and Biotechnology (BIOTEC)

Name: Siripong Thitamadee

Organization : Mahidol University. Faculty of Science

Email : siripong.thi@mahidol.ac.th

keyword: Penaeus vannamei

ThaSH: Shrimp culture

; Cellular immune response

ThaSH: Whiteleg shrimp -- Pathogenic microorganisms

ThaSH: Immune response

ThaSH: Phagocytosis

Abstract: In the era of the rapidly growing global population, intensive yet sustainable aquaculture is important for ensuring an adequate future supply of marine food. However, diseases have become the fundamental limiting factor for stable and reliable production in the shrimp aquaculture industry. The successful vaccination against white spot syndrome virus (WSSV) in shrimp by using recombinant envelope viral protein has been previously reported. To better understand the mechanism of the subunit vaccine, its effect on phagocytic activity was determined. The monomeric recombinant VP28 protein (1XrVP28) was prepared and injected into the shrimp at the concentration of 25 μg/shrimp. The hemolymph was collected at different time points; 15, 30, and 60 min, respectively to determine the total number of hemocyte (THC) and phagocytic activity. The results demonstrated that THC and phagocytic activity were significantly increased at 30 min post injection with 1XrVP28. But THC was declined at 60 min post injection. Our finding suggests that 1xrVP28 can be used to activate shrimp hemocytes and their phagocytic activity, which will be the baseline for optimizing the efficiency of vaccination used for shrimp to fight against WSSV.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2021

Modified: 2023-05-02

Issued: 2023-05-02

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BibliograpyCitation : In Thai Society for Biotechnology. Thai Society for Biotechnology and International Conference Online (TSB Online 2021) (pp.50-53). Bangkok : Thai Society for Biotechnology

eng

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