Anti-Hair Loss properties of resveratrol rich callus extract

Name: Wannisa Vichit

keyword: Anti-Hair Loss

MeSH: Baldness -- 35124

Classification :.DDC: e-Theses

; Callus

MeSH: Callus -- 301360

MeSH: Peanuts -- 97575

MeSH: Resveratrol -- 301361

MeSH: Cosmetics -- 301362

LCSH: Baldness -- 35124

LCSH: Callus -- 301360

LCSH: Peanuts -- 97575

LCSH: Resveratrol -- 301361

LCSH: Cosmetics -- 301362

Abstract: Resveratrol is a natural bioactive compound that has the potential to be used as an anti-hair loss active ingredient due to its ability to counteract age-related hair loss and stimulate new hair growth by acting as a 5-reductase inhibitor. The aim of this study was to investigate the anti-hair loss potential of resveratrol rich extracts of three plants: mahaad (MH), peanut (PN) and mulberry (MB) callus culture. MH, PN, and MB callus were induced on agar solidified Murashige and Skoog medium supplemented with 2 mg/ml 2,4-D, 1 mg/ml NAA, and 1 mg/ml BAP. Callus was a compact yellow texture with a high yield (60.03, 90.24, and 79.55% for MH, PN, and MB, respectively). The amount of containing resveratrol in callus was increased 1.1 – 1.7 folds in comparison to their other rich resveratrol part of plant; MH bark, PN sprout, and MB leaf. Callus was extracted with 95% ethanol to investigate the content of phenolic, resveratrol and antioxidant activities (ferric reducing power and DPPH radical scavenging) compared to their seed and other explants. All callus cultures provided significantly (p < 0.05) higher phenolic and resveratrol compounds than their seed and exhibited high effectiveness in FRAP and DPPH by 2 – 15 times. PN callus extract has the highest phenolic (0.42 ± 0.00 mg GAE/ml), resveratrol (1.92 ± 0.02 mg/g callus), FRAP (0.55 ± 0.01 mg AAE/ml), and DPPH (88.78 ± 0.72 %). The hair growth promoting effectiveness of extracts was investigated on human hair follicle dermal papilla cells by considering the potential effects on cytotoxicity, hair cell proliferation, 5-reductase inhibition, and growth factor expression. Based on the cytotoxicity curve, less than 2.5 %v/v of extracts displayed more than 90% cell survival which were selected to be used as a starting concentration for the hair cell activity analyses. The results showed that all extracts stimulated the proliferation of dermal papilla cells in a concentration-dependent manner, which the proliferative effect on DP cells related to prolonging the anagen phase. The extracts were also demonstrated to possess a potent inhibitory effect on 5α-reductase activity that was identified as a potential candidate for the treatment of androgen alopecia. PCR analysis showed that the extracts induced many growth factor genes, including IGF-1, KGF, HGF, and VEGF, suggesting that the hair growth effect of the extracts may be mediated through the regulation of growth factors. Overall, peanut callus extract was the most effective in cell proliferation (20.44 ± 0.78 %), 5α-reductase inhibition (27.83 ± 2.48 %), and gene regulation, especially IGF-1 and KGF. To evaluate stability and anti-hair loss efficacy of the extract, hair tonic base (F1) was developed and used as placebo, incorporation of 15% PN callus extract (F2), and 0.5% resveratrol (F3) as active ingredient. After 8-weeks of stability tests under cool (4°C), ambient (28°C), heat (45°C), and light conditions, F1 showed slightly changed in color. Only color of F2 storage in heat conditions was changed, while F3 in all storage conditions showed markedly changed. The ideal storage environment for preventing resveratrol degradation was low temperatures. A randomized, double-blind, placebo-controlled clinical trial of 60 volunteers were used to assess anti-hair loss efficacy. After 12 weeks of hair tonic application, F2 showed a significant reduction in daily hair shedding (66.98%) and hair fall by combing (83.92%) compared with the resveratrol group at 61.47% and 68.19%, respectively. Moreover, both formula F2 and F3 significantly increased the anagen/telogen ratios at 6 weeks of application. Adverse events and skin irritation were not reported during the study. Thus, the induction of callus can enhance hair growth by promoting the potential of plant tissue, which is interesting for use as an active ingredient in anti-hair loss products.

Mae Fah Luang University. Learning Resources and Educational Media Center

Address: CHIANG RAI

Email: library@mfu.ac.th

Name: Nisakorn Saewan

Role: Advisor

Created: 2022

Modified: 2023-04-28

Issued: 2023-04-28

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: e-Theses

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Cosmetic Science

Grantor: Mae Fah Luang University

©copyrights Mae Fah Luang University

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