Preservation of nicotine and solanesol from tobacco by cyclodextrins nanoencapsulation

Name: Sorrawee Chulurks

keyword: Nanoencapsulation

; Cyclodextrin

; βCD

; Inclusion complex

; Nicotine

; Solanesol

; Active compounds from tobacco

; MβCD

Abstract: Tobacco wastes contain major valuable active compounds which are nicotine and solanesol. Nicotine is widely used in pharmaceutical industries, especially for smoking cessation. There are several products for nicotine replacement therapy such as chewing gum, lozenges, and patches. Solanesol has many bioactivities such as antioxidant, anti-inflammatory, neuroprotective, and antimicrobial which can be used for pharmaceutical products. However, nicotine and solanesol instability caused by volatile substances, heat, presence of sunlight, or oxidizing agents, limits their usage. Therefore, the preservation of both active compounds is needed. The nanoencapsulation by using β-cyclodextrin (βCD) and methylated-β-cyclodextrin (MβCD) were investigated due to their non-toxicity. Computer simulation was used to predict the possibility of nicotine/CDs and solanesol/CDs inclusion complexes formation. The computer simulation results show the 1:1 and 2:1 host-guest molar ratio for nicotine/CDs and solanesol/CDs, respectively, which were further verified by experimental study. XRD, FTIR, TGA, and DSC results were analyzed to confirm the inclusion complexes formation. The preservation study of nicotine was performed at 25 ℃ and 40 ℃ for 21 days. The remaining amount of pure nicotine are 51.20% and 50.34% for 25 ℃ and 40 ℃, respectively. Nicotine/βCD and nicotine/MβCD inclusion complexes show the stability improvement of nicotine. The remaining amount of nicotine from nicotine/βCD and nicotine/MβCD inclusion complexes are 85.84% and 82.72% for 25 ℃ and 89.32% and 76.22% for 40 ℃, respectively. The preservation study of solanesol was performed at 25 ℃ and 40 ℃ for 28 days. The remaining amount of pure solanesol are 63.13% and 54.27% for 25 ℃ and 40 ℃, respectively. Solanesol/βCD and solanesol/MβCD inclusion complexes show the stability improvement of solanesol. The remaining amount of solanesol from solanesol/βCD and solanesol/MβCD inclusion complexes are 96.91% and 96.77% for 25 ℃ and 94.59% and 87.34% for 40 ℃, respectively. The results show that the nanoencapsulation technique using cyclodextrins can be performed to enhance the stability of both nicotine and solanesol when compared to the free forms which results in the shelf-life extension of nicotine and solanesol. Moreover, a skin permeation study shows the increment of permeated nicotine after being encapsulated by CDs. After 60 min, nicotine/βCD gel provides highest amount of permeated nicotine which is 13.57 μg followed by nicotine/MβCD gel, 9.81 μg, and pure nicotine gel, 0.95 μg. The improvement of nicotine skin permeation result can be applied for further use in the pharmaceutical industries

Thammasat University. Thammasat University Library

Address: BANGKOK

Email: preserv@tu.ac.th

Name: Luckhana Lawtrakul

Role: advisor

Created: 2020

Modified: 2023-02-13

Issued: 2023-02-13

วิทยานิพนธ์/Thesis

application/pdf

DOI: 10.14457/TU.the.2020.1028

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Engineering and Technology

Grantor: Thammasat University

©copyrights Thammasat University

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	10475sorrawee.pdf	3.03 MB

ใช้เวลา

0.033635 วินาที