Jennifer Tolentino Casabar. Enhancement of eazymatic hydrolysis potential through carbohydrate-binding module for efficient bioethanol production of pineapple fruit wastes. ปริญญาโท(Master Degree Master of Engineering Renewable Energy Enginee). . : มหาวิทยาลัยแม่โจ้, 2020.
Enhancement of eazymatic hydrolysis potential through carbohydrate-binding module for efficient bioethanol production of pineapple fruit wastes
Abstract:
Pineapple (Ananas comosus) is one of the leading fruits that is being processed, exported and consumed. Along with the processing of pineapple fruit and its consumption as a fresh fruit, tons of fruit by-products, such as fruit peel and crown are being produced each year. Value addition of these pineapple fruit wastes was done to maximize its worth and to address the problem in waste disposal of cannery companies. One additional value is the production of bioethanol from pineapple peel and crown due to its relatively high amount of sugars present. However, the application of naturally produced CBM from Trichoderma
harzianum has not yet been tested for bioethanol production. In this study, the effect of naturally produced CBM from T. harzianum in hydrolysis in the production of bioethanol from pineapple waste was evaluated. Three (3) varied pretreatments were used in the samples-- dilute acid, alkali and ultrasonic-mediated pretreatment. 2% cellulase, 2% T. harzianum and combination of cellulase and T. harzianum (C+T) in the hydrolysis process were used and optimized using Response Surface