Preparation of radiopharmaceutical TRODAT kit labeled with Tc-99m for diagnosis of Parkinson's disease

Name: Sudkanung Phumkem

Organization : Thailand Institute of Nuclear Technology (Public Organization). Radioisotope Center

Email : sudkanung@tint.or.th

Name: Siriwat Thida

Organization : Thailand Institute of Nuclear Technology (Public Organization). Radioisotope Center

Name: Sanguansak Hykunya

Organization : Thailand Institute of Nuclear Technology (Public Organization). Radioisotope Center

Name: Chonticha Phaophang

Organization : Thailand Institute of Nuclear Technology (Public Organization). Radioisotope Center

Name: Nawaporn Chareonsin

Organization : Thailand Institute of Nuclear Technology (Public Organization). Radioisotope Center

Name: Pattaporn Phathong

Organization : Thailand Institute of Nuclear Technology (Public Organization). Radioisotope Center

LCSH: Parkinson's disease

LCSH: Radiopharmaceuticals

Abstract: Patients with Parkinson’s disease show a decline in the density of the dopamine transporter (DAT) located in the striatum region of the brain. 99mTc-TRODAT-1, the first 99mTc tracer for imaging dopamine transporters in human was reported. This tracer showed the high affinity binding to DAT. This study aimed to prepare the radiopharmaceutical TRODAT lyophilized kit for the diagnosis of Parkinson’s disease. The freeze-dried formulation contained TRODAT- 1, SnCl2•2H2O, EDTA-Na2 and sodium glucoheptonate dihydrate. The lyophilized kit was radiolabelled by adding Tc- 99m 30-40 mCi/2 mL and heated for 30 min at 100°C by using a block heater, and subsequently cooled down to room temperature for 20 min. The radiochemical purity (RCP) of 99mTc-TRODAT was analyzed by using ITLC and HPLC. The RCP was more than 90% for 7 hours, and 99mTc-TRODAT had a pH of 4.7-5.0. The lyophilized product was sterile and a bacterial endotoxin test showed that the value was not more than the limit described in the USP. Biodistribution of 99mTc-TRODAT was performed in normal rats and found to have high uptake in the striatum area. These data indicated that the radiopharmaceutical of 99mTc-TRODAT had specific binding to the dopamine transporters (DAT) and therefore has the potential for use as a diagnostic radiopharmaceutical for Parkinson’s disease

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2022

Modified: 2022-11-24

Issued: 2022-11-24

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BibliograpyCitation : In King Mongkut's Institute of Technology Ladkrabang. Pure and Applied Chemistry International Conference (PACCON 2022) (pp.792-795). Bangkok : King Mongkut's Institute of Technology Ladkrabang

eng

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