Anti-inflammatory activity of the rhizomal extract and 4-methoxycinnamyl p-coumarate from etlingera pavieana in microglial cells

Name: Mayuree Poonasri

LCSH: Burapha University -- Faculty of Science

Classification :.DDC: Th 615.19

LCSH: Plant extracts

Abstract: Etlingera pavieana (Pierre ex Gagnep) R.M. Sm. is a Zingiberaceae plant. The rhizome is widely used as a traditional medicine and a spice. Previous studies have demonstrated that E. pavieana rhizomes and 4-methoxycinnamyl p-coumarate (MCC) isolated from its rhizomes show anti-inflammatory activity in mouse macrophages and human endothelial cells. Also, MCC has been demonstrated to exhibit in vivo anti-inflammatory activity in acute inflammation rat models. Nonetheless, the anti-inflammatory activities of E. pavieana rhizome extract and MCC in microglial cells have not yet been demonstrated. Therefore, in the present study, the ethanol extract (EPE) and MCC from E. pavieana rhizome were evaluated for their anti-inflammatory activity and the molecular mechanism underlying their anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated BV2 microglial cells. EPE and MCC were assessed for cytotoxicity on microglial cells by the MTT assay, and their non-toxic concentration was used to evaluate the anti-inflammatory activity. Anti-inflammatory activity was evaluated by using the production of nitric oxide (NO), prostaglandins E2 (PGE2) and tumor necrosis factor alpha (TNF-α) as indicators. The levels of crucial enzymes and key molecules in the nuclear factorkappa B (NF-κB) as well as the mitogen activated proteins kinase (MAPKs) signaling pathways were determined by qRT-PCR and Western blot analysis. The results showed that EPE remarkably reduced the production of NO and PGE2 in a concentration-dependent manner with IC50 value of 52.10±1.78 µg/mL and 37.32±6.92 µg/mL, respectively. EPE also decreased the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at both mRNA and protein levels. Furthermore, EPE suppressed NF-κB activation by attenuating the phosphorylation of nuclear factor-kappa B inhibitor alpha (IκBα) and NF-κB p65 subunit. In addition, MCC at non-cytotoxic concentrations considerably reduced both NO and PGE2 production in a concentration-dependent manner with an IC50 value of 3.32±1.13 µM and 13.32±2.18 µM, respectively. Also, MCC significantly suppressed the expression of iNOS and COX-2 at mRNA and protein levels. Additionally, MCC clearly reduced the production of TNF-α in a concentration-dependent manner with an IC50 value of 36.01±14.47 µM. Concomitantly, the expression of TNF-α mRNA level was discouraged by MCC. Moreover, MCC inactivated NF-κB signaling by decreasing the phosphorylation of IκBα and the NF-κB p65 subunit. Besides, MCC significantly attenuated the phosphorylation of p38 mitogen-activated kinase (p38 MAPK) and c Jun N terminal kinase (JNK). Conversely, the phosphorylation of extracellular signal regulated kinase (ERK) was significantly increased by MCC. In conclusion, the results suggest that EPE and MCC from E. pavieana rhizomes exert an anti-inflammatory activity in LPS-induced BV2 microglial cells. EPE suppressed NO and PGE2 production via blockade of the NF-κB signaling pathway. MCC also inhibited the production of pro-inflammatory mediators and cytokine through the NFκB and MAPKs signaling pathways. Accordingly, they might have the potential to be developed as functional food ingredients and dietary supplements for preventing neurodegenerative diseases.

Burapha University. Library

Address: CHONBURI

Email: buulibrary@buu.ac.th

Name: Klaokwan Srisook

Role: Principal advisor

Created: 2021

Modified: 2022-10-20

Issued: 2022-10-20

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: Th 615.19 Ma474A

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biochemistry

Grantor: Burapha University

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