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Autaipohn Kaikaew.. DNA binding activity of marine shrimp lvprofilin. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2022.
DNA binding activity of marine shrimp lvprofilin
Name: Yanisa Laoong-u-thai.
Organization :
Burapha University. Faculty of Engineering
Email :
yanisa@eng.buu.ac.th
Name: Warapond Wanna.
Organization :
Prince of Songkla University. Faculty of Science
Name: Autaipohn Kaikaew.
Organization :
Burapha University. College of Research Methodology and Cognitive Science
keyword:
Marine shrimp.
LCSH:
DNA-binding proteins.
LCSH:
Plants
-- Phylogeny.
Abstract:
Shrimp farming is an important business in Thailand and worldwide. The study of molecular biology and biochemical pathway of the key molecules controlling muscle growth is an essential to improve shrimp livestock. Profilin is a pivotal protein in muscle formation, especially actin protein. Its nuclear function has been reported in many species for gene regulation. Here in this work, we characterized the function of LvProfilin, a marine shrimp profilin from Litopenaeus vannamei, both in silico and in vitro. The phylogenetic tree of LvProfilin among organisms and its 3D protein structure showed that LvProfilin was highly conserved among shrimp and arthropods. The homology modeling of its 3D structure revealed 3 alpha-helices and 6 beta-strands similar to most eukaryotic profilins. To interpret its possible function, the gene expression of LvProfilin in various tissues was performed. We found that this gene was expressed in various tissues. This result may imply that LvProfilin could share a common function in all tissues. Nuclear activity has been a promising function of LvProfilin. We performed a DNA/RNA binding prediction analysis using DRNApred. The result indicated that Lysine-90 and Threonine-91 were the putative DNA-binding sites with the probability of 63.12 percent and 54.16 percent, respectively. Its binding activity was confirmed in vitro which bound stronger to single strand DNA than double strand DNA. To our best knowledge, this is the first report of DNA binding activity of profilin in invertebrates.
"
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2022
Modified:
2565-08-16
Issued:
2022-08-16
บทความ/Article
application/pdf
BibliograpyCitation :
APPLIED SCIENCE AND ENGINEERING PROGRESS. vol. 15, no. 3 (July-Sept. 2022), p. 1-11.
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.033849 วินาที
Yanisa Laoong-u-thai.
| Title | Contributor | Type |
|---|---|---|
| DNA binding activity of marine shrimp lvprofilin
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Yanisa Laoong-u-thai.;Warapond Wanna.;Autaipohn Kaikaew. | บทความ/Article |
Warapond Wanna.
| Title | Contributor | Type |
|---|---|---|
| DNA binding activity of marine shrimp lvprofilin
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Yanisa Laoong-u-thai.;Warapond Wanna.;Autaipohn Kaikaew. | บทความ/Article | |
| Antifungal activity of silver nanoparticles with the potential to control fungal contamination in the male inflorescences of palmyra palm
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Suchera Thananimit.;Pichayabha Sorsiw.;Warapond Wanna.;Souwalak Phongpaichit. | บทความ/Article |
Autaipohn Kaikaew.
| Title | Contributor | Type |
|---|---|---|
| DNA binding activity of marine shrimp lvprofilin
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Yanisa Laoong-u-thai.;Warapond Wanna.;Autaipohn Kaikaew. | บทความ/Article |