Abstract:
The aim of the experiment was to investigate effect of initial water temperature to survival rate of live sand worm (Perinereis sp.) during transportation. The trial was divided into 3 sub experiments. The First experiment consists of 4 initial water temperature (5, 10, 15 and 20oC) with testing period of 24 hrs. The other experiment had 3 initial water temperature (5, 10 and 20oC) with the testing period of 48 hrs. Triplicate groups of 500 g. sand worm were packed in plastic bags containing 1L of the designated water temperature before placing the bag into Styrofoam box containing ice to control inside temperature. With the testing period of 24 hrs., survival rates of sand worm packed in bag with initial water temperature of 5, 10, 15 and 20oC were 90.24, 90.15, 80.12 and 80.23%, respectively. Dissolved oxygen concentration in bag decreased with the increase of designated initial water temperature, but not below 4 mg/L. While total ammonia nitrogen and nitrite increased with the increase of designated initial water temperature with the highest values of 2.15 and 0.02 mg/L, respectively. With the testing period of 48 hrs., survival rates of sand worm packed in bag with initial water temperature of 5 and 20oC died at 42 hrs. Initial water temperature of 10˚C were 90.89% of survival rate. Dissolved oxygen concentration in bag with initial water temperatures of 10˚C were higher than 2 mg/L, but it was 0 mg/L in the initial water temperature of 5 and 20˚C. Total ammonia nitrogen and nitrite increased with the increase of initial water temperature and the highest concentrations were 2 and 0.03 mg/L, respectively and The third experiment was fill aeration in water. This experiment using water temperature starting at 10°C for 48 hours, it had 2 conditions in experiment were not fill aeration in water and fill aeration in water. The survival rate was 90.42 and 90.78%, but the dissolved oxygen in the fill aeration set was higher than not fill aeration set. Both water quality were good and did not affect for sand worm. It could be concluded that the optimum initial water temperature for live sand worm transportation was 10˚C because it made survival rate of sand worm high within transportation time not above 48 hrs. And if there is aeration in water, it will help increase the amount of dissolved oxygen.