The effect of rice-bran extract on fat metabolism in rats fed a high-fat diet

Name: Surasawadee Somnuk

keyword: Metabolic syndrome

; Rice bran High-fat diet

; Fat metabolism

Abstract: Rice bran water extract (RBE) is known for its nutritious bioactive compounds and was proven to decrease blood sugar and blood lipids, but little is known about its effect on the changes in fat metabolism. To help address this concern, the present study aim to investigate the effect of RBE from Khao Dawk Mali 105 or KDML 105 (Oryza sativa Linn) on hepatic fat metabolism. Male outbred Sprague-Dawley rats fed with high-fat diet were randomly divided into 2 groups including short-term (4weeks) and intermediate term (16weeks). After acclimatization, rats in short-term group were divided into 5 groups including 1) control (C, n = 16), 2) high-fat diet (HF, 65 % of energy derived from fat, n = 16), 3) HF + 2,205 mg/kg/day of RBE (HFR1, n = 16), 4) HF + 4,410 mg/kg/day of RBE (HFR2, n = 8) and 5) HF + metformin (9.5mg/kg/day for day 4 to day 16 and 19.1 mg/kg/day for the next 12 days (HFM, n = 8). After 4 weeks, half of animals in group 1 to 3 were continued for the treatment until the end of treatment period of 16 weeks (n = 8 / group). Weight, blood sugar, serum lipid profile, liver lipid content, histology of liver and epididymal fat pad were measured during 4 weeks and 16 weeks’ diet course. The expression of liver genes involved in fat metabolism and a regulator of liver inflammatory response were also assessed. After 4 weeks’ period, RBE treatment showed a significant decreased in body weight (BW), serum total cholesterol (TC), fasting blood sugar (FBS), area under the glucose curve (AUC), liver triglyceride and cholesterol contents. RBE also showed the fewer liver steatosis and abdominal fat deposition compared with that in HF fed rats. RBE treatment resulted to decrease in liver 3-hydroxy-3 methylglutaryl coenzyme A reductase (HMGCR) activities. Moreover, both mRNA and protein expression of sterol regulatory element binding protein-1 (SREBP-1) and nuclear factor kappa B (NF-B) genes were decreased, whereas the protein expression of adenosine monophosphate-activated protein kinase (AMPKα) gene was increased in RBE groups. For 16 weeks’ period, RBE treatment showed a significant decreased in serum TG, liver TC and also showed the fewer liver steatosis and abdominal fat deposition compared with that in HF fed rat. Moreover, RBE decreased the activity of HMGCR and the expression of NF-B genes. Although RBE exhibited no significant difference in the overall risk factors for metabolic markers, RBE aids in fat metabolism and helps lessen metabolic disturbances in blood and liver of rats fed with high-fat diet, partly by preventing the derangement of enzyme’s activity in cholesterol synthesis (HMGCR) and gene’s expression for fatty acid synthesis (SREBP1). In addition, RBE improves the expression of gene for fatty acid oxidation (AMPKα) and the expression of gene for inflammation (NF-B p65). Findings of this study, RBE is an alternative nutritional complement in preventive strategies against fat deposition and obesity associated complications

Thammasat University. Thammasat University Library

Address: BANGKOK

Email: preserv@tu.ac.th

Name: Nusiri Lerdvuthisopon

Role: advisor

Created: 2016

Modified: 2021-07-19

Issued: 2021-07-19

วิทยานิพนธ์/Thesis

application/pdf

DOI: 10.14457/TU.the.2016.1449

eng

DegreeName: Doctor of Philosophy

Level: Doctoral Degree

Descipline: Medical Sciences

Grantor: Thammasat University

©copyrights Thammasat University

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