Molecular biology effects of vanillin on keratinocytes

Name: Supak Taboonpong

keyword: Stemness

LCSH: Vanillin

; Cell adhesion

LCSH: Keratinocytes

; Oct-4

; Nanog

; Skin renewal

Abstract: Keratinocyte stem cells residing in the basal layer of the epidermis are thought to function in the process of the epidermis renewal and maintain the barrier property of the skin. This study has explored for the first time that vanillin, a natural compound from vanilla cured beans, has the ability to augment the stem cell property and signaling in the human HaCaT keratinocytes. The cell viability effect of vanillin on the keratinocytes was investigated by MTT assay. The western blot analysis was performed to determine the level of stem cell-mediated proteins, mesenchymal and epithelial markers. Results showed that treatment of the HaCaT cells with vanillin at non-toxic concentrations was able to significantly up-regulate the stemness mediators Oct-4, p-Oct-4 and Nanog. Besides, vanillin could increase the expression of epithelial adhesive protein E-cadherin, but has no significant effect on mesenchymal mediator Slug. As the stem cells as well as their stemness properties hold the central functions of skin renewal and repair, information gain from this study may benefit the development of vanillin to be used for skin therapy

Thammasat University. Thammasat University Library

Address: BANGKOK

Email: preserv@tu.ac.th

Name: Premjit Juntongjin

Role: advisor

Name: Pithi Chanvorachote

Role: co-advisor

Created: 2016

Modified: 2021-05-31

Issued: 2021-05-31

วิทยานิพนธ์/Thesis

application/pdf

DOI: 10.14457/TU.the.2016.539

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Dermatology

Grantor: Thammasat University

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