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Padchanee Sangthong. Microsatellite DNA analysis on autosome locus D20S482 of Thai population for human identification in forensic science. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2020.
Microsatellite DNA analysis on autosome locus D20S482 of Thai population for human identification in forensic science
Name: Ratchathikan Butsambo
Organization :
Chiang Mai University. Graduate School
Name: Ararat Jaiaree
Organization :
Chiang Mai University. Faculty of Science
Name: Padchanee Sangthong
Organization :
Chiang Mai University. Faculty of Science
Email :
padchanee.sangthong@cmu.ac.th
keyword:
Short tandem repeats
LCSH:
Thai
-- Population
Abstract:
Short tandem repeats (STRs) or microsatellite is a repeated sequence of nucleotides on the chromosome. The repeating patterns of STRs are varied among populations and are unique for each individual which can be used in personal identification. In this research, the study of the STR on the chromosome in D20S482 locus with AGAT repetition was investigated in Thai population. However, the frequency of each allele in D20S482 locus has not been reported in Thai population. This research aims to study and to analyze the repetitive sequence of microsatellite DNA on autosomal locus D20S482 in Thai population. Samples from 92 unrelated Thai individuals, which obtain Thai ethnicity for three generations and ages between 18 and 60 years old have been used. DNA has been extracted from the buccal cells and used as DNA templates for D20S482 amplification by polymerase chain reaction (PCR) technique. PCR products were determined by 10 percent PAGE with silver staining technique. The approximately 300 bp of PCR product fragments were separated by their sizes in PAGE, comparing to VC 50 bp DNA ladder marker. The DNA bands were cut and purified by NucleoSpin Gel and PCR Clean-up kit. Nucleotides sequencing were analyzed by the Big dye terminator technique and Biological sequence alignment editor (BioEdit) program version 7.2.5. The result revealed that the quality of genomic DNA was observed from 92 samples for used as DNA templates in the PCR reaction. The results showed that the repetitive patterns with AGAT tetranucleotides by 10, 11, 13, 14, 15 and 16 repeats were observed for D20S482 locus in Thai population. Therefore, the obtained result suggested that the D20S482 allelic ladder needs to be constructed and the genetic information can be applied in forensic science
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2020
Modified:
2021-05-31
Issued:
2021-05-31
บทความ/Article
application/pdf
BibliograpyCitation :
In Thai Society for Biotechnology. The 32nd Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2020) (pp.793-803). Bangkok : The Thai Society for Biotechnology
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.034231 วินาที
Ratchathikan Butsambo
| Title | Contributor | Type |
|---|---|---|
| Microsatellite DNA analysis on autosome locus D20S482 of Thai population for human identification in forensic science
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Ratchathikan Butsambo;Ararat Jaiaree;Padchanee Sangthong | บทความ/Article |
Ararat Jaiaree
| Title | Contributor | Type |
|---|---|---|
| Microsatellite DNA analysis on autosome locus D20S482 of Thai population for human identification in forensic science
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Ratchathikan Butsambo;Ararat Jaiaree;Padchanee Sangthong | บทความ/Article |
Padchanee Sangthong
| Title | Contributor | Type |
|---|---|---|
| Phylogenetic identification and cytotoxicity of Kaempferia parviflora extract
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Preeyaporn Klinjan;Pitchaya Mungkornasawakul;Yingmanee Tragoolpua;Padchanee Sangthong | บทความ/Article | |
| Microsatellite DNA analysis on autosome locus D20S482 of Thai population for human identification in forensic science
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Ratchathikan Butsambo;Ararat Jaiaree;Padchanee Sangthong | บทความ/Article | |
| PCR optimization for the amplification of mitochondrial DNA Positions 11,719-12,184 and preliminary evaluation for its potential to be applied for forensic applications in Thais
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Rattiyakorn Chattanon;Padchanee Sangthong;Worraanong Leewattanapasuk | บทความ/Article | |
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มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Kraikrit Utama;Nopawit Khamto;Puttinan Meepowpan;Paitoon Aobchey;Jiraporn Kantapan;Sittiruk Roytrakul;Padchanee Sangthong | บทความ/Article | |
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มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Phakorn Papan;Jiraporn Kantapan;Padchanee Sangthong;Puttinan Meepowpan;Nathupakorn Dechsupa | บทความ/Article | |
| Anti-breast cancer activity of 2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone and Its derivatives from Syzygium nervosum
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Pornthip Chawapun;Puttinan Meepowpan;Nathupakorn Dechsupa;Jiraporn Kantapan;Padchanee Sangthong | บทความ/Article |