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Padchanee Sangthong. Phylogenetic identification and cytotoxicity of Kaempferia parviflora extract. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2020.
Phylogenetic identification and cytotoxicity of Kaempferia parviflora extract
Name: Preeyaporn Klinjan
Organization :
Chiang Mai University. Faculty of Science
Organization :
Chiang Mai University. Faculty of Science
Name: Yingmanee Tragoolpua
Organization :
Chiang Mai University. Faculty of Science
Name: Padchanee Sangthong
Organization :
Chiang Mai University. Faculty of Science
Email :
padchanee.sangthong@cmu.ac.th
keyword:
Kaempferia parviflora
LCSH:
Flavonoids
LCSH:
Cladistic analysis
Abstract:
Kaempferia parviflora, also known as Thai ginseng, is a medicinal plant in the family Zingibeaceae. Phytochemicals of K. parviflora contain anti-inflammatory, anti-cancer, anti-bacterial, antioxidant, and anti-viral activities. In this research, we aim to evaluate phylogenetic, phytochemical identification and cytotoxicity of K. parviflora extracts in in vitro model. The plant extracts were prepared from dried rhizome using different extraction solvents such as hexane, ethyl acetate, dichloromethane, acetone, ethanol and methanol. Phylogenetic identification of K. parviflora was conducted using, two non-coding regions in chloroplast DNA, including trnL intron and trnL-F intergenic spacer, evaluated by polymerase chain reaction and direct DNA sequencing. The results revealed that PCR products of trnL intron and trnL-F intergenic spacer were approximately 600 and 400 bp, respectively. Nucleotide sequence analysis of K. parviflora sample showed 99.82 percent and 99.45 percent homology with reference sequences of K. parviflora in GenBank database in trnL intron and trnL-F intergenic spacer, respectively. For phytochemical analysis, the hexane extract of K. parviflora revealed the highest number of total polyphenols (153.39 ug GAE/g extract). On the other hand, methanolic extract contained the highest flavonoid contents at 176.81 ug QE/g extract. The cytotoxicities of the K. parviflora extracts were determined using MTT assay with various concentrations of the plant extracts (5 to 100 ug/mL). The methanolic extract showed low toxic than other extracts on mouse fibroblast L929 cell lines, with a IC50 value of 25.27 ug/mL.
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2020
Modified:
2021-05-31
Issued:
2021-05-31
บทความ/Article
application/pdf
BibliograpyCitation :
In Thai Society for Biotechnology. The 32nd Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2020) (pp.779-792). Bangkok : The Thai Society for Biotechnology
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.033525 วินาที
Preeyaporn Klinjan
| Title | Contributor | Type |
|---|---|---|
| Phylogenetic identification and cytotoxicity of Kaempferia parviflora extract
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Preeyaporn Klinjan;Pitchaya Mungkornasawakul;Yingmanee Tragoolpua;Padchanee Sangthong | บทความ/Article |
Pitchaya Mungkornasawakul
| Title | Contributor | Type |
|---|---|---|
| Phylogenetic identification and cytotoxicity of Kaempferia parviflora extract
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Preeyaporn Klinjan;Pitchaya Mungkornasawakul;Yingmanee Tragoolpua;Padchanee Sangthong | บทความ/Article |
Yingmanee Tragoolpua
| Title | Contributor | Type |
|---|---|---|
| Phylogenetic identification and cytotoxicity of Kaempferia parviflora extract
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Preeyaporn Klinjan;Pitchaya Mungkornasawakul;Yingmanee Tragoolpua;Padchanee Sangthong | บทความ/Article |
Padchanee Sangthong
| Title | Contributor | Type |
|---|---|---|
| Phylogenetic identification and cytotoxicity of Kaempferia parviflora extract
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Preeyaporn Klinjan;Pitchaya Mungkornasawakul;Yingmanee Tragoolpua;Padchanee Sangthong | บทความ/Article | |
| Microsatellite DNA analysis on autosome locus D20S482 of Thai population for human identification in forensic science
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