Treatment with a new bacteriophage isolated in Thailand attenuated salmonella enterica typhimurium colonization and dissemination in mice

Name: Chutikarn Sukjoi

Organization : Chiang Mai University. Faculty of Medicine

Name: Songphon Buddhasiri

Organization : Chiang Mai University. Faculty of Medicine

Name: Banyong Khantawa

Organization : Chiang Mai University. Faculty of Medicine

Name: Preeda Phothaworn

Organization : Mahidol University. Faculty of Medicine Siriraj Hospital

Name: Sunee Korbsrisate

Organization : Mahidol University. Faculty of Medicine Siriraj Hospital

Name: Parameth Thiennimitr

Organization : Chiang Mai University. Faculty of Medicine

Email : parameth@gmail.com, parameth.t@cmu.ac.th

LCSH: Salmonella

LCSH: Bacteriophages -- Therapeutic use

Abstract: Acute non-typhoidal salmonellosis (NTS) from the infection with the Gram negative bacterium Salmonella enterica Typhimurium (STM) is among the most common bacterial foodborne diseases worldwide. Human could get infected with the STM via a fecal-oral route transmission of contaminated food or drink. A life-threatening form of NTS called iNTS is rising due to the increasing rate of antibiotic resistance in the STM population. Bacteriophage therapy has been purposed as an alternative way for treatment of several antibiotic resistance bacterial infections including NTS. However, the specificity of bacteriophage on its bacterial host is an important factor for the success of a such therapy. Medical biotechnology for the development of Salmonella phage that can decrease an iNTS severity caused by Thai clinical isolated-STM (STMC) strains is crucial. Here, we aimed to investigate the anti-Salmonella activity of a new Salmonella phage, phiST-W77, isolated from a sewage water in Thailand. By using a phage spot assay and a mouse colitis model of an acute NTS, we found that a therapeutic administration of phiST-W77 inhibited growth of some STMCs and decreased gut colonization and systemic dissemination of STM in mice. Moreover, we found that phiST-W77 can persist in mouse gut without a tissue invasion. These indicate the high possibility to develop the phiST-W77 as a new Salmonella phage for treatment of antibiotic resistance iNTS patients in Thailand.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2020

Modified: 2021-05-28

Issued: 2021-05-28

บทความ/Article

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BibliograpyCitation : In Thai Society for Biotechnology. The 32nd Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2020) (pp.687-700). Bangkok : The Thai Society for Biotechnology

eng

©copyrights King Mongkut's University of Technology North Bangkok

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