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Nujarin Jongruja. Construction and expression of recombinant bacteriocin from bacillus subtilis. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2020.
Construction and expression of recombinant bacteriocin from bacillus subtilis
Name: Pacharavajee Duangkaew
Organization :
King Mongkut's University of Science and Technology Thonburi (KMUTT). Faculty of Science
Name: Nujarin Jongruja
Organization :
King Mongkut's University of Science and Technology Thonburi (KMUTT). Faculty of Science
Email :
nujarin.jon@kmutt.ac.th
keyword:
Bacteriocin
LCSH:
Anti-infective agents
LCSH:
Bacillus (Bacteria)
LCSH:
Foodborne diseases
Abstract:
At present, the problems encountered with antibiotic-resistant bacteria are increasing. So, the scientists are looking for natural substances to prevent these problems. The substance used is a bacteriocin produced by one bacterium to eliminate other bacteria to protect itself. In this research, we examined bacteriocin produced by Bacillus subtilis, by searching the bacteriocin database. A novel bacteriocin named LCI46 which is 195 base pair was designed and expressed in Escherichia coli BL21 production system. To our knowledge, no study has been conducted on this recombinant bacteriocin before. After preliminary experiment LCI46 displayed better expression and antimicrobial potential. Thus, the bacteriocin LCI46 was produced and purified. Tricine SDS-PAGE analysis of the purified bacteriocin showed a single band of peptide with molecular weight of 6.05 kDa. The highest expression was observed after 4 h induction. The purity of the LCI46-Histag was determined to be higher than 90 percent, with a final yield of 0.5 mg/l of LB broth. The recombinant LCI46 showed antibacterial activity against Gram positive (Staphylococcus aureus and Micrococcus luteus) bacteria with minimum inhibitory concentration (MICs) of 100 ug/ml and 50 ug/ml, respectively. Future studies should be performed to investigate other mechanism of actions of the LCI46
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2020
Modified:
2021-05-25
Issued:
2021-05-25
บทความ/Article
application/pdf
BibliograpyCitation :
In Thai Society for Biotechnology. The 32nd Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2020) (pp. 477-487). Bangkok : The Thai Society for Biotechnology
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.022326 วินาที
Pacharavajee Duangkaew
| Title | Contributor | Type |
|---|---|---|
| Construction and expression of recombinant bacteriocin from bacillus subtilis
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Pacharavajee Duangkaew;Nujarin Jongruja | บทความ/Article |
Nujarin Jongruja
| Title | Contributor | Type |
|---|---|---|
| Construction and expression of recombinant bacteriocin from bacillus subtilis
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Pacharavajee Duangkaew;Nujarin Jongruja | บทความ/Article | |
| Screening and application of microorganisms that degradation hydrocarbon compounds from oil stain removal plant
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Sudarat Rodpan;Nujarin Jongruja | บทความ/Article | |
| Isolation of probiotic bacteria from fermentation food and study properties to use prebiotics to promote probiotic bacteria growth
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Kantira Aincharoen;Nuttaworn Suppawongsanon;Nujarin Jongruja | บทความ/Article |