Cloning and characterization of a crustin gene from banana shrimp fenneropenaeus merguiensis (FmCrustin)

Name: Rachaya Leewanit

Organization : Prince of Songkla University. Faculty of Science

Name: Benjawan Thongkhluean

Organization : Prince of Songkla University. Faculty of Science

Name: Natthida Chaysawai

Organization : Prince of Songkla University. Faculty of Science

Name: Alwanee Yalapae

Organization : Prince of Songkla University. Faculty of Science

Name: Sarawut Chamnina

Organization : Prince of Songkla University. Faculty of Science

Name: Onnicha Rattanaporn

Organization : Prince of Songkla University. Faculty of Science

Email : onmicha.r@psu.ac.th

keyword: Fenneropenaeus merguiensis

LCSH: Peptide antibiotics

LCSH: Vibrio parahaemolyticus

Abstract: Antimicrobial peptides (AMPs) are the peptide that play an important role in the innate immune system. Crustins are invertebrate AMPs that found widely among crustaceans such as crabs, lobsters and shrimps. In the present study, a new crustin was identified from hemocytes of the banana shrimp Fenneropenaeus merguiensis (designated as FmCrustin). The full-length cDNA of FmCrustin was 491 bp, it contained a single open reading frame (ORF) of 366 bp that encodes a 121-amino acid protein comprising 17- amino acid signal peptide. FmCrustin is a type II crustin which contains a glycine-rich region, a cysteine-rich domain and a single whey acidic protein (WAP) domain. The predicted protein had a molecular weight and an isoelectric point (pI) of 12.55 kDa and 8.28, respectively. It shared 90.98 percent similarity with crustin-like antimicrobial peptide from F. indicus. The mRNA transcript of FmCrustin was detected in all examined tissues. The expression of FmCrustin in hemocytes was significantly upregulated after Vibrio parahaemolyticus infection. Therefore, our results suggested that FmCrustin may have important roles in the anti-bacterial immunity of F. merguiensis

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2020

Modified: 2021-05-21

Issued: 2021-05-21

บทความ/Article

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BibliograpyCitation : In Thai Society for Biotechnology. The 32nd Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2020) (pp. 410-418). Bangkok : The Thai Society for Biotechnology

eng

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