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Chakrit Tachaapaikoon. Cloning, expression and characterization of cellobiose 2-epimerase from Halocella sp. SP3-1 to produce epilactose. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2020.
Cloning, expression and characterization of cellobiose 2-epimerase from Halocella sp. SP3-1 to produce epilactose
Name: Sokhoeun Eat
Organization :
King Mongkut's University of Technology Thonburi. School of Bioresources and Technology
Name: Heng, Sobroney
Organization :
King Mongkut's University of Technology Thonburi. School of Bioresources and Technology
Name: Patthra Pason
Organization :
King Mongkut's University of Technology Thonburi. School of Bioresources and Technology
Name: Rattiya Waeonukul
Organization :
King Mongkut's University of Technology Thonburi. School of Bioresources and Technology
Name: Khanok Ratanakhanokchai
Organization :
King Mongkut's University of Technology Thonburi. School of Bioresources and Technology
Name: Chakrit Tachaapaikoon
Organization :
King Mongkut's University of Technology Thonburi. School of Bioresources and Technology
Email :
chakrit.tac@kmutt.ac.th
keyword:
Epimerization
LCSH:
Sweeteners
-- Synthesis
Abstract:
Epilactose (4-O-beta-D-galactopyranosyl-D-mannopyranose) is a rare disaccharide derived from lactose (4-O-beta-D-galactopyranosyl-Dglucopyranose). It is regarded as a new prebiotic and low-calorie sweetener. Epilactose can be produced both chemically and enzymatically. It is more convenient and greener to synthesize epilactose from lactose by the enzymatic approach using cellobiose 2-epimerase (CE; EC 5.1.3.5) as a single biocatalyst in a one-step reaction without any co-substrates. CE is an attractive enzyme because that can produce epilactose from lactose via 2-epimerization of the reducing end of sugar residue. Here, we described a CE from Halocella sp. SP3-1 (HaCE) as a first report. The open reading frame of HaCE contained nucleotides of 1,200 bp, corresponding to 400 amino acids, with a calculated molecular weight of 47 kDa. A putative HaCE gene was cloned and expressed in Escherichia coli BL21 (DE3). The optimum temperature and pH of the recombinant HaCE (rHaCE) were 40 Celsius degrees and 7.0, respectively. Moreover, the rHaCE revealed activity with lactose and cellobiose, but not maltose, sucrose, and trehalose as substrate. These properties of rHaCE from Halocella sp. SP3-1, thus, makes it a promising candidate for use in the dairy industry
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2020
Modified:
2021-05-20
Issued:
2021-05-20
บทความ/Article
application/pdf
BibliograpyCitation :
In Thai Society for Biotechnology. The 32nd Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2020) (pp.289-301). Bangkok : The Thai Society for Biotechnology
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
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0.03499 วินาที
Sokhoeun Eat
| Title | Contributor | Type |
|---|---|---|
| Cloning, expression and characterization of cellobiose 2-epimerase from Halocella sp. SP3-1 to produce epilactose
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Sokhoeun Eat;Heng, Sobroney;Patthra Pason;Rattiya Waeonukul;Khanok Ratanakhanokchai;Chakrit Tachaapaikoon | บทความ/Article |
Heng, Sobroney
| Title | Contributor | Type |
|---|---|---|
| Cloning, expression and characterization of cellobiose 2-epimerase from Halocella sp. SP3-1 to produce epilactose
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Sokhoeun Eat;Heng, Sobroney;Patthra Pason;Rattiya Waeonukul;Khanok Ratanakhanokchai;Chakrit Tachaapaikoon | บทความ/Article |
Patthra Pason
| Title | Contributor | Type |
|---|---|---|
| Isolation and identification of ligninolytic bacillus albus PA3/3 from fermented tea leaf and its application in industrial wastewater treatment
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Khin, Pan Myint Myat;Chakrit Tacchapaikoon;Rattiya Waeonukul;Kanok Rattanakanokchai;Patthra Pason | บทความ/Article | |
| Cloning, expression and characterization of cellobiose 2-epimerase from Halocella sp. SP3-1 to produce epilactose
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Sokhoeun Eat;Heng, Sobroney;Patthra Pason;Rattiya Waeonukul;Khanok Ratanakhanokchai;Chakrit Tachaapaikoon | บทความ/Article |
Rattiya Waeonukul
| Title | Contributor | Type |
|---|---|---|
| Isolation and identification of ligninolytic bacillus albus PA3/3 from fermented tea leaf and its application in industrial wastewater treatment
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Khin, Pan Myint Myat;Chakrit Tacchapaikoon;Rattiya Waeonukul;Kanok Rattanakanokchai;Patthra Pason | บทความ/Article | |
| Cloning, expression and characterization of cellobiose 2-epimerase from Halocella sp. SP3-1 to produce epilactose
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Sokhoeun Eat;Heng, Sobroney;Patthra Pason;Rattiya Waeonukul;Khanok Ratanakhanokchai;Chakrit Tachaapaikoon | บทความ/Article |
Khanok Ratanakhanokchai
| Title | Contributor | Type |
|---|---|---|
| Cloning, expression and characterization of cellobiose 2-epimerase from Halocella sp. SP3-1 to produce epilactose
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Sokhoeun Eat;Heng, Sobroney;Patthra Pason;Rattiya Waeonukul;Khanok Ratanakhanokchai;Chakrit Tachaapaikoon | บทความ/Article | |
| Screening of lignocellulose degrading fungi with decolorizing ability
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Damkerng Bundidamorn;Lakha Salaiphet;Taweerat Vichitsoonthonkul;Khanok Ratanakhanokchai;Paripok Phitsuwan | บทความ/Article |
Chakrit Tachaapaikoon
| Title | Contributor | Type |
|---|---|---|
| Cloning, expression and characterization of cellobiose 2-epimerase from Halocella sp. SP3-1 to produce epilactose
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Sokhoeun Eat;Heng, Sobroney;Patthra Pason;Rattiya Waeonukul;Khanok Ratanakhanokchai;Chakrit Tachaapaikoon | บทความ/Article |