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Kittipong Rattanaporn. The effects of glucose and alternative inducers on the growth and the extracellular production of recombinant b-mannanase of Escherichia coli KMAN-3. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2020.
The effects of glucose and alternative inducers on the growth and the extracellular production of recombinant b-mannanase of Escherichia coli KMAN-3
Name: Kanok-on Khongcharoen
Organization :
Kasetsart University. Fermentation Technology Research Center
Name: Kanin Ratchatapaiboon
Organization :
Kasetsart University. Fermentation Technology Research Center
Name: Tanat Uan-On
Organization :
Kasetsart University. Fermentation Technology Research Center
Name: Preuk Thangpromphan
Organization :
Kasetsart University. Fermentation Technology Research Center
Name: Kittipong Rattanaporn
Organization :
Kasetsart University. Fermentation Technology Research Center
Email :
kittipong.r@ku.th
keyword:
Beta-Mannanase
LCSH:
Fermentation
LCSH:
Escherichia coli
LCSH:
Galactose
Abstract:
The aims of this study were to investigate the effects of glucose and the effects of alternative inducers on the growth of Escherichia coli KMAN- 3 and the extracellular production of recombinant B-mannanase. The recombinant E. coli KMAN-3 cells were cultured in the modified M9 medium containing 0.05 percent w/v glucose compared with the modified M9 medium without the addition of glucose under the IPTG induction at 4 hours post inoculation. The results showed that the cell density (OD600nm) of E. coli KMAN-3 obtained from the culture in the modified M9 medium containing glucose provided higher cell density of 1.668 than the culture in the glucose-free M9 medium. However, the maximum B-mannanase activity of the extracellular enzyme was obtained at the level of 90.31+6.48 U/ml from the culture in the glucose-free M9 medium. In contrast, the enzyme activity obtained from the culture in glucosecontaining M9 medium (85.18+1.39 U/ml) was significantly lower than the culture in the glucose-free medium because of the catabolite repression. According to the investigation of an alternative inducer, lactose and galactose were used to auto-induction of the recombinant mannanase production. It was found that the culture of E. coli KMAN-3in the glucose-free M9 medium with lactose auto-induction showed the maximum specific growth rate of 0.58+0.09 h-1. While the maximum B- mannanase activity was obtained from the culture of E. coli KMAN-3 in the glucose-free M9 medium with the induction of IPTG and galactose resulted in the enzyme activity of 90.31+6.48 U/ml and 72.31+3.85 U/ml, respectively. Therefore, it could conclude that the addition of glucose in the modified M9 medium could support only the growth of E. coli KMAN 3; whereas, it could not increase the extracellular production of recombinant mannanase. Moreover, galactose could be an alternative inducer for recombinant mannanase production of E. coli KMAN-3 in the modified M9 medium
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2020
Modified:
2021-05-17
Issued:
2021-05-17
บทความ/Article
application/pdf
BibliograpyCitation :
In Thai Society for Biotechnology. The 32nd Annual Meeting of the Thai Society for Biotechnology and International Conference (TSB 2020) (pp.15-26). Bangkok : The Thai Society for Biotechnology
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.04466 วินาที
Kanok-on Khongcharoen
| Title | Contributor | Type |
|---|---|---|
| The effects of glucose and alternative inducers on the growth and the extracellular production of recombinant b-mannanase of Escherichia coli KMAN-3
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Kanok-on Khongcharoen;Kanin Ratchatapaiboon;Tanat Uan-On;Preuk Thangpromphan;Kittipong Rattanaporn | บทความ/Article |
Kanin Ratchatapaiboon
| Title | Contributor | Type |
|---|---|---|
| The effects of glucose and alternative inducers on the growth and the extracellular production of recombinant b-mannanase of Escherichia coli KMAN-3
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Kanok-on Khongcharoen;Kanin Ratchatapaiboon;Tanat Uan-On;Preuk Thangpromphan;Kittipong Rattanaporn | บทความ/Article |
Tanat Uan-On
| Title | Contributor | Type |
|---|---|---|
| The effects of glucose and alternative inducers on the growth and the extracellular production of recombinant b-mannanase of Escherichia coli KMAN-3
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Kanok-on Khongcharoen;Kanin Ratchatapaiboon;Tanat Uan-On;Preuk Thangpromphan;Kittipong Rattanaporn | บทความ/Article |
Preuk Thangpromphan
| Title | Contributor | Type |
|---|---|---|
| The effects of glucose and alternative inducers on the growth and the extracellular production of recombinant b-mannanase of Escherichia coli KMAN-3
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Kanok-on Khongcharoen;Kanin Ratchatapaiboon;Tanat Uan-On;Preuk Thangpromphan;Kittipong Rattanaporn | บทความ/Article |
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