แจ้งเอกสารไม่ครบถ้วน, ไม่ตรงกับชื่อเรื่อง หรือมีข้อผิดพลาดเกี่ยวกับเอกสาร ติดต่อที่นี่ ==>
หากไม่มีอีเมลผู้รับให้กรอก thailis-noc@uni.net.th
หากไม่มีอีเมลผู้รับให้กรอก thailis-noc@uni.net.th
Sudarma Dita Wijayanti. Electrochemical Characterization and Hybridization Efficiency of Aminated DNA Probes Immobilized on Screen Printed Carbon Electrodes. Master's Degree(Biotechnology). King Mongkut's University of Technology Thonburi. KMUTT Library.. : King Mongkut's University of Technology Thonburi, 2010.
Electrochemical Characterization and Hybridization Efficiency of Aminated DNA Probes Immobilized on Screen Printed Carbon Electrodes
Name: Sudarma Dita Wijayanti
keyword:
Screen Printed Carbon Electrode
Abstract:
The research has focused on the how to attach DNA strands to carbon screen printed
electrodes via an amino group, so that they stand up with a good orientation. This is to
ensure the full length of the probe is available for hybridization. Electrodeposition at -1.0 V
and covalent attachment of the aminated DNA probes by l-ethyl-3-(3
dimethylaminopropyl)-carbodiimide (EDC) and N- Hydroxysuccinimide (NHS). The DNA
coverages and hybridization efficiencies were determined using both chronocoulometry and
UV absorption. The use of propylamine as a spacer for the DNA strands, the effect of the
propylamine : DNA ratio and the effect of the probe length were also examined. The best
results were gained by immobilising pDNA-15 spacing with propyl amine spacer at a 0.5
mole fraction ratio. Maximum ssDNA coverages determined by chronocoulometry were
1.37x10 13 molecules cm-2 obtained for electrodeposition and 4.97x10 12 molecules cm-2 for
covalent attachment. The hybridization efficiencies ranged from 39.9 % to 80.3 %. These
coverages were lower than those obtained by UV absorption. Measurement by UV -vis
absorption does not involve the charge compensation assumptions required by the
chronocoulometric method. The affinity constant (Ka) for duplex formation for the probes
immobilized by covalent attachment (1.52x10 8 Mol) was higher than for the
electrodeposition method (1.51x10 7 M-1). Presumably, covalent attachment gave the
ssDNA better orientation on the electrode in terms of allowing hybridization.
King Mongkut's University of Technology Thonburi. KMUTT Library.
Address:
BANGKOK
Email:
info.lib@mail.kmutt.ac.th
Name: Werasak Surareungchai
Role:
Advisor
Created:
2010
Modified:
2011-04-16
Issued:
2011-04-01
วิทยานิพนธ์/Thesis
application/pdf
CallNumber:
BIT622
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Biotechnology
©copyrights King Mongkut's University of Technology Thonburi
RightsAccess:
ใช้เวลา
0.027015 วินาที
Sudarma Dita Wijayanti
| Title | Contributor | Type |
|---|---|---|
| Electrochemical Characterization and Hybridization Efficiency of Aminated DNA Probes Immobilized on Screen Printed Carbon Electrodes
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี Sudarma Dita Wijayanti | Werasak Surareungchai | วิทยานิพนธ์/Thesis |
Werasak Surareungchai