Gita Rosidah. Effects of Food Preservatives and Ozonated Water on Microbial Decontamination and Quality of Fresh Cut Cabbage (Brassica oleraceae L.). Master's Degree(Biotechnology). King Mongkut's University of Technology Thonburi. KMUTT Library.. : King Mongkut's University of Technology Thonburi, 2010.
Effects of Food Preservatives and Ozonated Water on Microbial Decontamination and Quality of Fresh Cut Cabbage (Brassica oleraceae L.)
Abstract:
The objective of this study was to investigate the effects of potassium sorbate (PS),
ozonated water or their combinations on postharvest microbial decontamination and the
qualities of fresh cut cabbage. Cabbage slices were dipped into sterile water (control), PS
solutions at 5 and 10 g/L for 1 and 2 min. All treated samples were then packed in PET box
and stored for 5 days at 4 C. The results revealed that the dipping of PS at 5 g/L for 2 min
was the best treatment to reduce coliforms and total bacteria by 0.50 - 0.30 and 0.22 - 0.17
log CFU/g FW from day 3 until day 5 of shelf life respectively, it also maintained the
texture of fresh cut cabbage, meanwhile total chlorophyll content and browning degree were
not significant different among all treatments. The effect of ozonated water on microbial
decontamination and the qualities of fresh cut cabbages was conducted. Cabbage slices were
treated with sterile water (control) and ozonated water, which generated by ozone generator
with 2,500 mglhr of the capacity, for 5 and 10 min. The concentrations of ozone in water
were 0.014 and 0.057 mgO3/L respectively. All treated samples were then stored for 5 days
at 4 C. The results showed that ozonated water for 5 min reduced total bacteria by 0.95 only
in day 1 of shelf life and coliforms on fresh cut cabbage about 4.30 - 0.22 log CFU/g FW
during 5 days of shelf life. Ozonated water for 5 min also maintained the texture of fresh cut
cabbage throughout 5 days of shelf life, whereas weight loss, browning index and total
chlorophyll content were not significant different in all treatments. The combined treatment
between PS solution followed by ozonated water was investigated during storage at 4?C.
The results showed that ozonated water alone or its combination could reduce total bacteria
on fresh cut cabbage by 0.84 - 0.36 and 1.29 - 0.77 log CFU/g FW during 9 days of shelf
life, while yeasts and molds was reduced by 4.08 - 0.74 and 2.65 - 0.38 log CFU/g FW
days during 7 of shelf life respectively. Ozonated water treatment alone showed the
significant reduction of coliforms count by 3.63 - 0.71 log CFU/g FW for 7 days of shelf
life. Both of ozonated water and the combined treatments could maintain the texture and
delayed weight loss of fresh cut cabbage, while total chlorophyll content was not significant
different among all treatments. This result implies that use of ozonated water alone was
sufficient to decontaminate the microbial in fresh cut cabbage without any negative effects
on the qualities. The appropriate cycle numbers of ozonated water (5 L), used for
decontaminating coliforms, total bacteria and yeasts and molds in 250 g of fresh cut
cabbage, were 3, 1 and 1 cycles, respectively.
King Mongkut's University of Technology Thonburi. KMUTT Library.