Utilization of cassava pulp and cassava wastewater for bioethanol production

Name: Tawida Srimuang

Organization : Siam Technology College. Faculty of Health Sciences

Email : tawidas@siamtechno.ac.th

Name: Supawadee Polprasert

Organization : Mahidol University. Faculty of Public Health

keyword: Cassava Pulp

ThaSH: Cassava Wastewater

ThaSH: Enzymatic Hydrolysis

ThaSH: Bioethanol Fermentation

Abstract: The purpose of this study was to optimize the bioethanol production from cassava pulp and cassava wastewater. The study was divided into two phase: 1.) Pretreatment and enzymatic hydrolysis 2.) Bioethanol fermentation. The effect of substrate and ratio of loading 1, 3, 5, and 7% (w/v) with the mixed enzyme α-amylase and glucoamylase ratio of 1:1 (v/v).The results showed that at 7% substrate loading, the highest reducing sugar concentration was 89.55±1.13 g/L. Regarding, the best ratio of α-amylase and glucoamylase at 1:1 (v/v) with fermentation time of 4 h gave the highest yield of 0.58±0.02 (g product/g substrate). In addition, the highest glucose yield of 0.36±0.04 (g product/g substrate) was observed in 2 h fermentation time after hydrolysis with α-amylase and glucoamylase, using cellulase and xylanase ratio of 1:1 (v/v). The results indicated that usage of mixed enzyme proved to spend shorter hydrolysis time and enhance the fermentable sugar production from cassava waste. The enzymatic hydrolysate was used as substrate for bioethanol fermentation, using S.cerevisiaeTISTR5339. The results showed that the ethanol yield at fermentation time at 6 h with S.cerevisiae5, 10, 20 and 30% (v/v) were 0.27±0.027, 0.23±0.002, 0.19±0.022 and 0.16±0.011 (g ethanol/g substrate), respectively. The result indicated that ethanol yield decreased with increasing cell concentration of S.cerevisiaeTISTR5339 from 5% (v/v) to 30% (v/v).

Rajamangala University of Technology Phra Nakhon. Office of Academic Resources and Information Technology

Address: BANGKOK

Email: arit@rmutp.ac.th

Created: 2562

Modified: 2020-09-20

Issued: 2020-09-07

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BibliograpyCitation : RMUTP Research Journal, Vol. 13, No. 2, July-December 2019, pp.167-175

eng

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