Antioxidant activitles of bioactive compounds from barrington augusta kurz. and syzygium gratum (WIGHT) S.N. Mitra VAR. gratum in ban ang-ed official community forest project (The chaipattana foundation)

Name: Wilailuck Leamklang

LCSH: Burapha University.Faculty of Science

Abstract: Barrington augusta Kurz. (Jig) and Syzygium gratum (Wight) S.N. Mitra var. gratum (Sameddang) are found in the Eastern region of Thailand. They are indigenous plants collected from Ban Ang-Ed Official Community Forest Project, Chantaburi province. They have been used in healthcare prevention several diseases by traditional doctors. In addition, a previous report of both plants in hot water extract showed high antioxidant activities, but there is still no study of the antioxidant activity of bioactive compound from these plants. Therefore, this work aims to evaluate the bioactive compounds by determining the total phenolic content, and the antioxidant activity from sub-extracts and sub-fractions after purification via TLC and column chromatography compared with the crude extract. The results showed that water extract of B. augusta had the highest total phenolic contents of 267.589 ± 1.544 mg GAE/g extract, and it could strongly inhibit DPPH radical (EC50 = 0.052 ± 0.005 mg/mL). On the other hand, ethyl acetate extract expressed the greatest FRAP value of 434.340 ± 1.344 mg TE/g extract and showed ferrous ion-chelating activity of 83.18%. From three assays, it indicated that ethyl acetate fraction exhibited the high antioxidant activities. Therefore, we decided to choose this extract to further isolate by column chromatography. After isolation, the percent of DPPH radical scavenging activity of active fractions from B. augusta (38.38%) was lower than crude ethyl acetate extract (65.67%). Moreover, both of FRAP value and ÿrrous ion-chelation were decreased to 144.287 ± 1.273 mg TE/g extract and 36.79%, respectively. For the result of S. gratum, ethyl acetate extract had the highest total phenolic contents with 151.458 ± 1.360 mg GAE/g extract, and it could v remarkably inhibit DPPH radical (EC50 = 0.071 ± 0.002 mg/mL). In addition, it was also expressed the highest FRAP value of 890.885 ± 4.724 mg TE/g extract and showed ferrous ion-chelating activity of 30.43%. The results suggested that the ethyl acetate extract showed high antioxidant activity. Thus, this extract was selected to further isolate by column chromatography. After isolation, active fractions from S. gratum had still high DPPH radical scavenging activity (94.36%) and FRAP value of 767.373 ± 3.272 mg TE/g extract. The ÿrrous ion-chelating activity of active fraction seemed to be lower (39.11%) than its crude extract. From those results indicate that B. augusta and S. gratum were showed remarkable antioxidant activity in ethyl acetate extract, and active compound can provide additional information for identification and characterization the bioactive compounds. For the future, their bioactive compounds can be applied in nutritional and dietary supplemented applications with their antioxidant activities and total phenolic contents.

Burapha University. Library

Address: CHONBURI

Email: buulibrary@buu.ac.th

Name: Chatchawin Petchlert

Role: Principal advisor

Name: Ekaruth Srisook

Role: Co-advisor

Created: 2018

Modified: 2020-06-16

Issued: 2020-06-16

ÇÔ·ÂÒ¹Ô¾¹¸ì/Thesis

application/pdf

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biological Science

Grantor: Burapha University

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