Inhibitory effect of Thai vegetable extracts on activity of lipase, alpha-amylase, trypsin and alcohol dehydrogenase

Name: Adchara Sangchan

LCSH: Burapha University.Faculty of Science

Abstract: Obesity is a major public health risk causing diabetes, hypertension, and cardiovascular disease. In general, taking medication is the most popular approach to weight loss because it takes less time, yet patients can lose more weight than other methods. However, prescription drugs can also bring about deadly adverse effects. An alternative way to lose weight is to reduce lipase and alpha-amylase activities in the digestive system. Thus, this research aimed to study in vitro inhibitory effect of extracts from 30 Thai vegetables on the activities of digestive enzymes (pancreatic lipase, pancreatic alpha-amylase, and trypsin) and an enzyme in alcohol metabolism (alcohol dehydrogenase). Local Thai vegetables were sampled from three different seasons, including summer, spring and winter, and were extracted with water, ethanol or hexane at 37 °C or at 90°C. Each extract was subsequently assayed for enzyme inhibition using Orlistat®, Acarbose®, phenylmethylsulfonyl fluoride (PMSF), or nicotinic acid as positive controls. Results indicated that crude extracts of Acacia pennata (Cha om), Apium graveolens ( Khuen-chai) , Citrus hystrix DC. ( Makrut lime) , Ocimum tenuiflorum (Kaphrao), Pandanus amaryllifolius (Bai toei) and Tiliacora triandra (Ya nang) possessed compelling anti-lipase activities as their IC50 (the half maximum inhibitory concentration) were in the test range (< 300 µg/ml). Furthermore, crude extracts of A. pennata (Cha om), A. graveolens (Khuen-chai), Allium tuberosum (Kow choi), Coccinia grandis (Tum lung), Leucaena glauca (Kra thin), Mentha cordifolia (Saranae), P. amaryllifolius (Bai toei), T. triandra (Ya nang) and Zingiber officinale (Khing) also demonstrated significant anti-alpha amylase activities. Interestingly, crude v extracts of Allium sativum (Kra tium), Alpinia galanga (Khaa), M. cordifolia (Saranae), Piper sarmentosum (Chaphlu) and Salanum torvum (Ma khuea phuang) embraced considerable anti-trypsin activities, implying that these extracts might not be suitable for weight loss due to an interference with protein digestion. Moreover, crude extracts of A. galangal ( Khaa), A. graveolens (Khuen-chai) and Z. officinale ( Khing) had additional anti-alcohol dehydrogenase activities, suggesting that these extracts would interfere with other drug metabolisms in our body, therefore the application should be handled with care. Surprisingly, there were partial relationships between phenolic content of the extracts and their anti-lipase activities, indicating that active constituents exerting anti-lipase activities in most Thai vegetable extracts were, in part, phenolic compounds. Taken together, the favorable Thai vegetables and their extracts recommended for functional food development and natural anti-obesity remedy are A. pennata (Cha om), P. amaryllifolius (Bai toei), and T. triandra (Ya nang) based on their specific anti-lipase and anti-alpha amylase activities and their high phenolic content.

Burapha University. Library

Address: CHONBURI

Email: buulibrary@buu.ac.th

Name: Salil Chanroj

Role: Principal advisor

Created: 2018

Modified: 2020-06-16

Issued: 2020-06-16

วิทยานิพนธ์/Thesis

application/pdf

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biological Science

Grantor: Burapha University

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