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Vo Phong Vu Anh Tuan. Cloning and Sequencing of Sialoadhesin and CD163 cDNA from Porcine Reproductive and Respiratory Syndrome virus infected Porcine Alveolar Macrophages. Master's Degree(Veterinary Medicine). Chulalongkorn University. Office of Academic Resources. : Chulalongkorn University, 2011.
Cloning and Sequencing of Sialoadhesin and CD163 cDNA from Porcine Reproductive and Respiratory Syndrome virus infected Porcine Alveolar Macrophages
การโคลนนิ่งและการศึกษาลำดับเบสซีดีเอ็นเอของตัวไวรับไซอะโลแอดฮีซินและซีดี 163 ที่แยกได้จากแมคโครฟาจสุกรที่ติดเชื้อไวรัสพีอาร์อาร์เอส
Name: Vo Phong Vu Anh Tuan
LCSH:
Macrophages
LCSH:
Swine
-- Virus diseases
Abstract:
Sialoadhesin (Sn) and CD163 receptors were reported as two essential receptors on porcine alveolar macrophages (PAM) for PRRSV infection and importance for the study of the immunity of pigs after infection. The objectives of this study were cloning and sequencing the porcine Sn (full and N-terminal domain) and CD163 (full and domain 5) cDNA from 3 strains of PRRSV infected PAM of Thai nursery pigs and comparison of gene expression level with GAPDH gene as internal reference. In this thesis, total RNA was extracted from 6 positive EU, US and HP-PRRSV infected PAM. The porcine Sn (full and N-terminal domain) and CD163 (full and domain 5) cDNA were further amplified using PCR technique. Band densitometry was used to quantify expression level. Then, the cDNA were cloned into pCR®-XL-TOPO® (full cDNA) and pCR®8-GW-TOPO® (N-terminal domain and domain 5 cDNA) vectors. The nucleotide and deduced amino acid sequences including compositions were then analyzed and compared. The results showed that 10 clones of Sn (full) and 20 clones of CD163 (full) were constructed in pCR®-XL-TOPO® vectors, while 24 clones of Sn (N-terminal domain) and 29 clones of CD163 (domain 5) were transferred into pCR8®-GW-TOPO® vectors. The sequences and compositions of nucleotide and deduced amino acids of these genes from 3 strains of PRRSV were successfully analyzed and showed more than 95% similarity. Furthermore, the US strain of PRRSV infected PAM collected from Thai nursery pigs showed higher expression level of porcine Sn and CD163 cDNA than that of the HP and EU strains.
Chulalongkorn University. Office of Academic Resources
Address:
BANGKOK
Email:
cuir@car.chula.ac.th
Name: Phong Vu Anh Tuan.
Role:
advisor
Name: Athipoo Nuntaprasert
Role:
advisor
Created:
2011
Modified:
2020-04-28
Issued:
2020-04-28
วิทยานิพนธ์/Thesis
application/pdf
URL: http://cuir.car.chula.ac.th/handle/123456789/60416
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Veterinary Medicine
Grantor: Chulalongkorn University
©copyrights Chulalongkorn University
RightsAccess:
ใช้เวลา
0.02169 วินาที
Vo Phong Vu Anh Tuan
| Title | Contributor | Type |
|---|---|---|
| Cloning and Sequencing of Sialoadhesin and CD163 cDNA from Porcine Reproductive and Respiratory Syndrome virus infected Porcine Alveolar Macrophages
จุฬาลงกรณ์มหาวิทยาลัย Vo Phong Vu Anh Tuan | Phong Vu Anh Tuan. Athipoo Nuntaprasert | วิทยานิพนธ์/Thesis |
| Effect of recombinant chimeric PKR-APAF-1 protein on PRRS virus infected MARC-145 cell line
จุฬาลงกรณ์มหาวิทยาลัย Vo Phong Vu Anh Tuan | Athipoo Nuntaprasert Achara Tawatsin | วิทยานิพนธ์/Thesis |
Phong Vu Anh Tuan.
| Title | Creator | Type and Date Create |
|---|---|---|
| Cloning and Sequencing of Sialoadhesin and CD163 cDNA from Porcine Reproductive and Respiratory Syndrome virus infected Porcine Alveolar Macrophages
จุฬาลงกรณ์มหาวิทยาลัย ;Phong Vu Anh Tuan.;Athipoo Nuntaprasert | Vo Phong Vu Anh Tuan | วิทยานิพนธ์/Thesis |
Athipoo Nuntaprasert
| Title | Creator | Type and Date Create |
|---|---|---|
| Cloning and sequencing of 3ABC gene of foot and mouth disease virus type O from a Thai pig isolate from Ratchaburi province in 2005
จุฬาลงกรณ์มหาวิทยาลัย Athipoo Nuntaprasert | Kulisara Marupanthorn | วิทยานิพนธ์/Thesis |
| Cloning and Sequencing of Sialoadhesin and CD163 cDNA from Porcine Reproductive and Respiratory Syndrome virus infected Porcine Alveolar Macrophages
จุฬาลงกรณ์มหาวิทยาลัย ;Phong Vu Anh Tuan.;Athipoo Nuntaprasert | Vo Phong Vu Anh Tuan | วิทยานิพนธ์/Thesis |
| Effect of recombinant chimeric PKR-APAF-1 protein on PRRS virus infected MARC-145 cell line
จุฬาลงกรณ์มหาวิทยาลัย Athipoo Nuntaprasert;Achara Tawatsin | Vo Phong Vu Anh Tuan | วิทยานิพนธ์/Thesis |