Study of Morphology and Effect of Anti-transpirants on Stomatal Aperture and Quality of Patumma (Curcuma alismatifolia x Curcuma cordataladdawan) Inflorescence
Abstract:
The stomata of Patumma inflorescence (Curcuma alismatifolia x Curcuma cordata laddawan)
consists of two subsidiary cells that each one locates beside the guard cell without coverage.
Stomatal type called typical stomata is the guard cells arranged line of epidermis cells. The coma
bract and stems of inflorescence had no significant difference in stomatal density. Each coma bract,
divided into 3 parts; tip, middle and base of coma bract, was found the highest stomatal density at
tip, middle and base, respectively (224.17, 109.23 and 33.60 stomata/cm3). The coma bracts were
observed the higher stomatal numbers at adaxial leaf than abaxial leaf (176.47 and 68.20
stomata/cm3). Moreover, trichomes were found at the tip of both pink and green coma bract. High
relative humidity reduced stomatal aperture and increased stomatal closured numbers. Stomatal
aperture and stomatal opening were reduced by 5 and 10 uM abscisic acid (ABA) and methy
jasmonate (MeJA), causing the reduction of transpiration rate. But they did not delay loss of fresh
weight. Patumma inflorescences held in 5 uM of MeJA solution increased water uptake and
transpiration rate as compared to others. Score of quality of acceptance of inflorescences at the end
of vase life that Patumma inflorescence placed in distilled water was accepted higher than
treatments. Neither ABA nor MeJA prolong the vase life of inflorescences vase solution contained
5 uM ABA or 5 uM MeJA + 15 mg/l HQS + 2% sucrose increased stomatal closures and reduced
stomatal aperture in comparison to distilled water, but did not extend the vase life and increase the
quality of Patumma inflorescences.