Watcharee Sopanowong. Comparative evaluation of different extraction method for biological compounds and antioxidant activity from young citrus maxima MERR. for cosmetic utilization. Master's Degree(Cosmetic Science). Mae Fah Luang University. Learning Resources and Educational Media Center. : Mae Fah Luang University, 2019.
Comparative evaluation of different extraction method for biological compounds and antioxidant activity from young citrus maxima MERR. for cosmetic utilization
Abstract:
In general, young fruits of pomelo are trimmed off and abandoned as agricultural waste during cultivation. To increase the value of those wastes, therefore, this research aimed to investigate the effects of extraction solvents on the biological activities of young fruits by the maceration technique. In this study, four different maceration solvent systems were used. The four systems were 95% ethanol, 75% ethanol, 50% ethanol, and propylene glycol, respectively. The age of young fruits used in this study was in three ranges: one-month-old, two-months-old, and three-months-old. The Folin-Ciocalteu reagent assay and aluminum chloride colorimetric method were used for measuring phytochemical constituents content. Antioxidant activity of the extract was measured by DPPH radical scavenging assay and hydrogen peroxide scavenging assay. The result in this study showed that one-month-old pomelo fruit extracted by 75% ethanol had the highest total phenolic content (31.64 ± 0.39 mg gallic acid equivalent/mL extract) and the highest total flavonoids content (5.41 ± 0.47 mg quercetin equivalent/mL extract). This specimen also had the strongest antioxidant activities. The extract had DPPH radical scavenging activity of 179.07 ± 8.48 mg ascorbic acid equivalent/mL extract and hydrogen peroxide scavenging activity of 124.63 ± 0.74 mg ascorbic acid equivalent/mL extract.
The summary showed that the phytochemical constituents and antioxidant activities decreased corresponding to the maturing of the fruits. One-month-old fruit extracted by 75% ethanol was shown the highest phytochemical constituents and antioxidant activity. Further studies on phytochemical constituents, quantitative analysis, standardization, and formulation into cosmetic products should be conducted.
Mae Fah Luang University. Learning Resources and Educational Media Center