Glucose palmitate and glucose palmate synthesis catalyzed by carica papaya latex lipase

Name: Ninnapat Tiensri

Address: 333 Moo1, Thasud, Muang, Chiang Rai 57100

Organization : Mae Fah Luang University. School of Cosmetic science. Cosmetic Science

Email : ask.library@mfu.ac.th

keyword: Carica papaya

LCSH: Carica

Classification :.LCCS: QU145

ThaSH: Esterification

ThaSH: Plant Preparations -- therapeutic use

ThaSH: Lipase

ThaSH: Fatty Acids

Abstract: This study was purposed to synthesize the glucose palmitate and glucose palmate catalyzed by Carica papaya latex lipase (CPL) and investigate the factors affecting to the synthesis reactions. The CPL was prepared from water-insoluble part of the C. papaya latex and enzyme powder obtained with 7.05±0.10 % yield. The CPL showed the highest activities at temperature of 35˚C and pH value between 8.0-9.0. It possessed the highest pH stability at 8.0-9.0 and temperature of 35°C . The CPL did not require solvent rinsing before catalysis. The CPL-catalyzed glucose palmitate synthesis in n-butanol was performed. The optimum condition composed of glucose: palmitic acid ratio of 1:1 in 10 ml of n-butanol with 10% of water content, 7.84% of molecular sieve with shaking at 170 rpm, 35°C for 72 h demonstrated the highest conversion of 94.71±1.00 %. TLC and FT-IR results confirmed the presence of this sugar fatty acid ester which possessing HLB value of 5.60 and the critical micelle concentration (CMC) of 1.14×10-6 M. Moreover, the CPL could be reusable and still remained its catalytic activity of 85% after 4 recycles. The glucose palmate synthesis from glucose and Morakot palm oil catalyzed by the CPL was performed in solvent-free system. Fatty acid compositions of palm oil identified by GC-MS were found to be oleic acid (51.77±0.67%), palmitic acid (37.22±0.48 %), stearic acid (6.01±0.61%), myristic acid (2.20±0.29 %) and lauric acid (0.74±0.07%) as major components. The glucose palmate synthesis was determined its optimum condition by using response surface methodology (RSM). Seventeen synthesis conditions were generated by Box-Behnken design and the products were further determined by UPLC. The condition of 50°C, reaction time of 48 h, and 1,200 U of CPL amount was the best condition and possessed the highest conversion of 30.05±0.26 %. The transesterification between Morakot palm oil and glucose was confirmed by TLC and FT-IR. The obtained sugar fatty acid ester revealed HLB value of 6.20 and the CMC of 3.16×10-9 M. This study has proved that the natural immobilized lipase or CPL potentially provided catalytic ability for natural surfactant synthesis by using palm oil and glucose as the substrates. Moreover, this transesterification can be performed without using any solvent. Therefore, the enzymatic natural surfactant synthesis using Thai agricultural products could be promising as an alternative method for further industrial application, which were environmentally friendly and could increase the value of Thai agricultural products.

มหาวิทยาลัยแม่ฟ้าหลวง. ศูนย์บรรณสารและสื่อการศึกษา

Address: เชียงราย

Email: library@mfu.ac.th

Name: Phanuphong Chaiwut

Role: Advisor

Email : ask.library@mfu.ac.th

Name: Punyawatt Pintathong

Role: Co-Advisor

Email : ask.library@mfu.ac.th

Created: 2019

Issued: 2563

Modified: 2020-02-24

Issued: 2020-02-24

วิทยานิพนธ์/Thesis

application/pdf

CallNumber: Thesis QU145 N715g 2019

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Cosmetic Science

Grantor: Mae Fah Luang University

©copyrights Mae Fah Luang University

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