Synbiotics with the Capability on Alteration of Gastrointestinal Microbiota and their Activities of Colon Cancer Induction in Rats

ซินไบโอติกส์ที่มีความสามารถในการปรับเปลี่ยนจุลินทรีย์และกิจกรรมของจุลินทรีย์ของระบบทางเดินอาหารในหนูที่ถูกเหนี่ยวนำให้เกิดมะเร็งลำไส้ใหญ่

Name: Thanawat Pattananandecha

LCSH: Microorganisms

Classification :.DDC: 579

LCSH: Gastrointestinal system

LCSH: Intestine, Large|xCancer

Abstract: Colon cancer is the third most common cancer in the world, diet is an important cause of the cancer. Synbiotics, a combination of probiotic and prebiotic bacteria, have been suggested to reduce the risk of colon cancer. Intestinal microbiota consists of diverse, dynamic and complex populations and they have crucial role in overall health of the host. Positive regulation of intestinal microbiota leads to health promotion of host that can be achieved by prebiotics. Jerusalem artichoke (JA) is known as inulin-rich, an innate polysaccharide, plant, approximately 14 - 20 % of fresh weight accounts for inulin content. Acid hydrolyzed inulin from JA with different DP, InuL (DPav = 23) and InuS (DPav = 18) were studied as carbon sources for the growth of Lactobacillus plantarum, growth inhibition of Escherichia coli and Salmonella enterica serovar Typhi evaluated by co-culture and agar well diffusion methods. The results suggested that InuL and InuS support the growth of L. plantarum at significant level (p < 0.05) compared to control and it improves the bacterial inhibitory nature of L. plantarum against E. coli and S. Typhi. Six months of sub-chronic toxicity test was conducted on the safety of InuL and InuS with 1, 10 and 30% diet. The results showed that the inulins both InuL and InuS are safe for male and female rats with no mortality and abnormal behavior (feeding and drinking, excretion, sleeping, cohabitation and responsiveness) and no effect on weight gain, feed intake, hematological and clinical chemistry parameter, organs weights. The impact of InuL and InuS with respect to intestinal microbiota and their activities in azoxymethane (AOM)-induced preneoplastic aberrant crypt foci (ACF) rats were studied. Rats were received 10% of InuL or InuS along with diet for 17 weeks. At the end of the experiment, cecal contents of rats were examined for selected microbiota, organic acids, putrefactive compounds, microbial enzymes and ACF formation. The results suggested that inulin diets significantly increased the weight and decreased the pH of the cecal content. The rats fed with InuL increased the population of Lactobacillus spp. and Bifidobacteria, and decreased E. coli and S. enterica serovar Typhi population. Inulin supplementation significantly decreased the cecal phenol, p-cresol and indole and reduced the activity of β-glucuronidase, azoreductase and nitroreductase. InuL had significantly reduced the ACF formation than InuS. InuL was selected to combine with L. paracasei to prepare synbiotic and studied its’ impact with respect to intestinal microbiota and their activities in azoxymethane (AOM)-induced preneoplastic aberrant crypt foci (ACF) rats were studied. The results revealed that the synbiotic intervention protected the AOM-mediated colon cancer than either probiotic (L. plantarum) or prebiotic (InuL) alone by increasing the probiotic population, organic acids, reducing the pathogen load, microbial enzymes, putrefactive compounds, and ACF formation. Synbiotic powder were prepared using freeze drying method. L. paracasei was freeze dried with inulin JA (hydrolyzed inulin from Jerusalem artichoke, DPav = 23), Inulin GR (Chicory inulin, DPav > 10), Inulin SC (DPav = 8), Inulin FF (DPav = 16), skim milk, maltodextrin, sorbitol, and trahalose at 2.5 - 10%. The results showed that the trahalose significantly protected the survivability of L. paracasei at 79.9% while Inulin JA showed only 33.1% of survival. The selected freeze dried synbiotic powders were studied for its viability when passage through the in vitro stomach and intestine model, and during storage. Freeze dried L. paracasei with 7.5% inulin JA showed the high survival rate of L. paracasei during passage through SGF (96.1%) while freeze dried L. paracasei with 5.0 % inulin FF showed survival rate of 75.5% during passage through both SGF and SIF. Freeze dried L. paracasei with 7.5% of trehalose that kept at 4 °C had the high survival rate of 91.6% while freeze dried L. paracasei with 5.0% maltodextrin had the high shelf life at 25 °C for 30 days (42.3%), whereas the survival of L. paracasei with others protectants were not detectable. Among L. paracasei with inulins, L. paracasei with 7.5% inulin JA had the high survival rate of 59.2% when stored at 4 °C for 60 days.

Chiang Mai University Library

Address: Chiang Mai

Email: cmulibref@cmu.ac.th

Name: Nantarit Chokethaworn

Role: Chairman

Name: Chaiyavat Chaiyasut

Role: Member

Name: Sasithorn Sirilun

Role: Member

Name: Prasit Suwannalert

Role: Member

Name: Chutinun Prasitpuriprecha

Role: Member

Created: 2016

Modified: 2020-01-09

Issued: 2020-01-09

วิทยานิพนธ์/Thesis

application/pdf

Th 579 T367S

eng

DegreeName: Doctor of Philosophy

Level: Master's Degree

Descipline: Pharmacy

Grantor: Chiang Mai University

©copyrights Chiang Mai University

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