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Pelden, Sonam.. The role of trefoil factor family-1 (TFF1) in estrogen-promoted apoptosis resistance in breast cancer cells. Master's Degree(Immunology). Mahidol University. Mahidol University Library and Knowledge Center. : Mahidol University, 2012.
The role of trefoil factor family-1 (TFF1) in estrogen-promoted apoptosis resistance in breast cancer cells
Name: Pelden, Sonam.
MeSH:
TFF1 protein, human.
MeSH:
Breast Neoplasms.
MeSH:
Apoptosis.
Abstract:
TFF1 is a secreted protein and is expressed in various types of carcinomas. The TFF1 gene contains an estrogen responsive element in its promoter, due to which its expression could be regulated by estrogen. In addition, estrogen has demonstrated its ability to promote resistance to doxorubicin in estrogen receptor positive MCF-7 breast cancer cell line. This study aims to explain the estrogen induced resistance to chemotherapy by exploring the relationship between the estrogen-induced TFF1 and its role in inferring resistance to apoptosis. Permanent knockdown of TFF1 gene in MCF-7 cell was generated, to test the sensitivity to doxorubicin in comparison to the parental cell in the presence or absence of 17β-estradiol, which is a potent estrogenic agent. Flow cytometry analysis showed that, 17β-estradiol could rescue MCF-7 cells from doxorubicin induced apoptosis (P = 0.016) but failed to demonstrate this effect in the TFF1 knockdown MCF-7 cells. Trypan blue cell counting showed that, neutralizing secreted TFF1 resulted in aggravated apoptosis, much higher than just the doxorubicin treated condition (P = 0.029). Moreover reconstituting TFF1 in the knockdown cells refurbished its protective role (P = 0.05). Apoptosis protein array revealed changes in expressions of key apoptosis regulatory proteins under varying treatment conditions, particularly the anti-oxidative enzymes catalase and heme oxygenase 1 (HO-1), on the whole trying to restore homeostasis. These phenomena determine the contribution of TFF1 in estrogen-promoted resistance to apoptosis induced by doxorubicin in MCF-7 breast cancer cells. TFF1 may be a target for manipulation of chemotherapeutic resistance in breast cancer
Mahidol University. Mahidol University Library and Knowledge Center
Address:
NAKHONPATHOM
Email:
liwww@mahidol.ac.th
Name: Peti Thuwajit
Role:
Thesis Advisors
Name: Chanitra Thuwajit
Role:
Thesis Advisors
Name: Pornchai O-Charoenrat
Role:
Thesis Advisors
Created:
2012
Modified:
2019-11-12
Issued:
2019-11-12
วิทยานิพนธ์/Thesis
application/pdf
CallNumber:
TH P381r 2012
eng
DegreeName: Master of Science
Level: Master's Degree
Descipline: Immunology
Grantor: Mahidol University
©copyrights Mahidol University
RightsAccess:
ใช้เวลา
0.2556 วินาที
Pelden, Sonam.
| Title | Contributor | Type |
|---|---|---|
| The role of trefoil factor family-1 (TFF1) in estrogen-promoted apoptosis resistance in breast cancer cells
มหาวิทยาลัยมหิดล Pelden, Sonam. | Peti Thuwajit Chanitra Thuwajit Pornchai O-Charoenrat | วิทยานิพนธ์/Thesis |
Peti Thuwajit
Chanitra Thuwajit
Pornchai O-Charoenrat
| Title | Creator | Type and Date Create |
|---|---|---|
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มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Utomporn Phalavonk;Gareth Clayton;Pornchai O-Charoenrat | Thoranin Intarajak | วิทยานิพนธ์/Thesis |
| The role of HER2 oncogene in head and neck cancer
มหาวิทยาลัยมหิดล Pornchai O-Charoenrat;Piyawadee Leksrisakul | Hathainan Sirikett | วิทยานิพนธ์/Thesis |
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มหาวิทยาลัยมหิดล Pornchai O-Charoenrat;Tuenjai Chuangsuwanich;Suwattanee Kooptiwutl;Thawornchai Limjindaporn | Doonyapat Sa-nguanraksa | วิทยานิพนธ์/Thesis |
| The role of trefoil factor family-1 (TFF1) in estrogen-promoted apoptosis resistance in breast cancer cells
มหาวิทยาลัยมหิดล Peti Thuwajit;Chanitra Thuwajit;Pornchai O-Charoenrat | Pelden, Sonam. | วิทยานิพนธ์/Thesis |