Abstract:
The objective of this research was to study active ingredients of mungbean and sprout extracts using three different types of solvents: distilled water, ethanol and ethyl acetate to study bioactive substances content, antioxidant activity and anti-tyrosinase activity. The experimental results showed that the maximum extract yield (%) from mungbean was obtained with ethanol (4.66 ± 0.99%) meanwhile the maximum extract yield (%) from sprout was obtained with distilled water (11.43 ± 1.43%). Mungbean extraction with distilled water produced the maximum total phenolic content (TPC) (342.52 ± 19.20 mg GAE/g extract) and sprout extraction with ethyl acetate (667.41 ± 17.17 mg GAE/g extract). Mungbean extraction with ethanol produced the maximum total flavonoids content (TFC) (69.81 ± 1.07 mg QE/g extract) and sprout extraction with ethyl acetate (179.17 ± 21.67 mg QE/g extract). The highest antioxidant activity of mungbean extraction with distilled water (1143.50 ± 76.67 mg AAE/g sample or 288.57 ± 73.85 mg TEAC/g sample) and sprout extraction with ethyl acetate (1164.06 ± 56.85 mg AAE/g sample or 308.37 ± 54.76 mg TEAC/g sample). Mungbean extraction with ethanol produced the highest anti-tyrosinase activity (443.35 ± 2.64 mg Kojic acid/g sample) and sprout extraction with ethanol (1187.24 ± 81.63 mg Kojic acid/g sample).
From the development of skin care products indicated that the researcher selected 0.2% concentration of mungbean and sprout extracts in serum products because this level of concentration gave less intense smell and color than the formula containing 0.6% extract. The volunteers accepted physical characteristics of serum. Volunteers satisfaction score after using the serum, It could be concluded that a serum with the highest level of satisfaction was sprout extract serum was 4.14 ± 0.51., followed by mungbean extract serum was 4.03 ± 0.61, and basic serum was 4.03 ± 0.50, respectively with a statistical significance level of 0.05. In terms of studying the efficiency of skin moisturizing of products in the opinions of 26 volunteers after 4 week usage, it was found that three types of products gave high skin moisture. Mungbean extract serum had the highest level of skin moisture (183.7 ± 91.20). It could be concluded that mungbean extract serum could produce higher skin moisture of users than basic serum and sprout extract serum, respectively. There was no difference in skin moisture among three types of products with a statistical significance level of 0.05.