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Pimchai Chaiyen. Characterization of 2-oxo-hept-3-ene-1,7-dioic acid hydratase from Acinetobacter baumannii. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2019.
Characterization of 2-oxo-hept-3-ene-1,7-dioic acid hydratase from Acinetobacter baumannii
Name: Wachirawit Chinantuya
Organization :
Mahidol University. Faculty of Science
Name: Pirom Chenprakon
Organization :
Mahidol University. Institute for Innovative learning
Name: Ruchanok Tinikul
Organization :
Mahidol University. Faculty of Science
Email :
ruchanok.tin@mahidol.ac.th
Name: Pimchai Chaiyen
Organization :
Vidyasirimedhi Institute of Science and Technology. Department of Biomolecular Science and Engineering
keyword:
Acinetobacter baumannii
LCSH:
Bacteria
-- Identification
LCSH:
Aromatic compounds
Abstract:
Hydratase or hydro lyase catalyzes the hydration of alkene to yield an alcohol product. As reaction catalyzed by hydratase has high regio and chemo selectivities, the enzyme is therefore attractive for using as biocatalyst in synthesizing of stereo specific compounds required in pharmaceutical ingredients. We have previously identified the putative gene encoding a hydratase in the p hydroxyphenylacetate (4 HPA) operon that likely catalyzes water addition to 2 oxo hepta 3 ene 1,7 dioic acid (OHED) to form 2,4 dihydroxy hept 2 ene 1,7 dioic acid. In this study, the OHED hydratase from Acinetobactor baumannii (AbOHED) expression was optimized in E. coli and the result showed that AbOHED gave the highest yield of expression in auto induction media at 25 Celsius degrees for 18 hr. The enzyme was then purified by ammonium sulfate precipitation at 10 30 percent and DEAE Sepharose anion exchange column. As the AbOHED substrate is not commercially available, the AbOHED activity was tested with substrate analogues containing carbon double bond, including fumaric acid and ferulic acid and product was identified by HPLC MS. The result showed that AbOHED was able to convert fumaric acid to malic acid, but was unable to use ferulic acid. Therefore, fumaric acid will be used as a substrate for further AbOHED characterization. The knowledge of AbOHED properties will pave the ways for the enzyme applications
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2019
Modified:
2019-08-30
Issued:
2019-08-30
บทความ/Article
application/pdf
BibliograpyCitation :
In Chemical Society of Thailand under the Patronage of Professor Dr. HRH Princess Chulabhorn. Pure and Applied Chemistry International Conference 2019 (PACCON 2019) (CB26-31). Bangkok : Mahidol University
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.028631 วินาที
Wachirawit Chinantuya
| Title | Contributor | Type |
|---|---|---|
| Characterization of 2-oxo-hept-3-ene-1,7-dioic acid hydratase from Acinetobacter baumannii
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Wachirawit Chinantuya;Pirom Chenprakon;Ruchanok Tinikul;Pimchai Chaiyen | บทความ/Article |
Pirom Chenprakon
| Title | Contributor | Type |
|---|---|---|
| Characterization of 2-oxo-hept-3-ene-1,7-dioic acid hydratase from Acinetobacter baumannii
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Wachirawit Chinantuya;Pirom Chenprakon;Ruchanok Tinikul;Pimchai Chaiyen | บทความ/Article |
Ruchanok Tinikul
| Title | Contributor | Type |
|---|---|---|
| Characterization of 2-oxo-hept-3-ene-1,7-dioic acid hydratase from Acinetobacter baumannii
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Wachirawit Chinantuya;Pirom Chenprakon;Ruchanok Tinikul;Pimchai Chaiyen | บทความ/Article |
Pimchai Chaiyen
| Title | Contributor | Type |
|---|---|---|
| Theoretical study of oxidative half-reaction mechanism of p-Hydroxyphenylacetate 3-Hydroxylase (HPAH)
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Surawit Visitsatthawong;Pimchai Chaiyen;Panida Surawatanawong | บทความ/Article | |
| Overexpression of pyranose dehydrogenase in E.coli platform
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Pangrum Punthong;Litavadee Chuaboon;Jeerus Sucharitakul;Pimchai Chaiyen;Thanyaporn Wongnate | บทความ/Article | |
| Expression and characterization of novel aldehyde deformylating oxygenase from Pseudomonas plecoglossicida
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Supacha Buttranon;Juthamas Jaroensuk;Jeerus Sucharitakul;Pimchai Chaiyen;Thanyaporn Wongnate | บทความ/Article | |
| Characterization of 2-oxo-hept-3-ene-1,7-dioic acid hydratase from Acinetobacter baumannii
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Wachirawit Chinantuya;Pirom Chenprakon;Ruchanok Tinikul;Pimchai Chaiyen | บทความ/Article |