Development and validation of a method for determination of ethyl glucuronide and ethyl sulfate in postmortem blood by liquid chromatography tandem mass spectrometry

Name: Thammitanan Loungyot

Organization : Chiang Mai University. Faculty of Medicine

Name: Anongphan Junkuy

Organization : Chiang Mai University. Faculty of Medicine

Name: Pongruk Sribunditmongkol

Organization : Chiang Mai University. Faculty of Medicine

Name: Chaturong Kanchai

Organization : Chiang Mai University. Faculty of Medicine

Email : chaturong.k@cmu.ac.th

keyword: Ethyl glucuronide

LCSH: Diethyl sulfate

LCSH: Liquid chromatography -- Methodology

LCSH: Tandem mass spectrometry

ThaSH: Blood -- Analysis

Abstract: Ethyl glucuronide (EtG) and ethyl sulfate (EtS) are non-oxidative metabolites of ethanol that can be used as specific biomarkers of alcohol intake. This study aims to develop and validate a liquid chromatography tandem mass spectrometric method for determination of EtG and EtS in blood samples and to apply it to analysis of postmortem blood samples. Sample preparation was performed by protein precipitation with methanol, the supernatant was evaporated to dryness and then reconstituted with distilled water. Analytes of interest were separated on a Luna 5 μm C18(2) column with gradient elution mode. This method provided good linearity for EtG (0.05-10 microgram per mL) and EtS (0.005-5 microgram per mL). LOD and LOQ were 0.04 and 0.05 microgram per mL for EtG and 0.002 and 0.005 microgram per mL for EtS, respectively. There was no significant matrix effect. Data for accuracy, precision, recovery and process efficiency were corresponded with the international guidelines. This method has been employed for analysis of blood ethanol-positive obtained from autopsy cases. The results revealed that these markers were most negative in low blood ethanol concentration samples. This protocol was simple and specific to simultaneous determination of EtG and EtS. It is a useful tool for investigation of the alcohol origin in forensic toxicology task

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2019

Modified: 2019-08-29

Issued: 2019-08-29

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BibliograpyCitation : In Chemical Society of Thailand under the Patronage of Professor Dr. HRH Princess Chulabhorn. Pure and Applied Chemistry International Conference 2019 (PACCON 2019) (AC121-127). Bangkok : Mahidol University

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