Improvement of microbial quality and antioxidant activity of Centella asiatica (L.) Urban by electron beam irradiation

Name: Panchalee Prakhongsil

Organization : Thailand Institute of Nuclear Technology (Public Organization)

Email : punpanchalee@hotmail.com

Name: Wachiraporn Pewlong

Organization : Thailand Institute of Nuclear Technology (Public Organization)

Name: Surasak Sajjabut

Organization : Thailand Institute of Nuclear Technology (Public Organization)

Name: Sirilak Chookaew

Organization : Thailand Institute of Nuclear Technology (Public Organization)

keyword: Centella asiatica (L.)

LCSH: Antioxidants

; Bai-Bua-Bok

LCSH: Microbial diversity

; Electron beam irradiation

LCSH: Phenolic compounds

Abstract: Centella asiatica (L.) Urban, locally known in Thai as Bai-Bua-Bok, has a wide range of biological activities that are considered health benefits such as anti-inflammatory, wound healing, and antioxidant activities. In this study, samples of C. asiatica leaf powder were irradiated by electron beam at doses from 0-20 kGy before evaluated for their total phenolic content, antioxidant activities, and any remaining bacteria or fungi contaminants. The total aerobic microbial count and the total fungi count of the unirradiated sample were found to be 6.80 x 107 and 6.30 x 106 CFU/g, respectively. Pathogenic Clostridium spp. (per 10 g) was also found. Electron beam irradiation, at a dose of 7 kGy was effective in reducing the microbial load in C. asiatica as indicated in microbial limit test, whereas a dosage of 20 kGy was needed for complete decontamination. The levels of the total phenolics, DPPH scavenging activities and ferric reducing antioxidant potentials of the unirradiated sample and the irradiated samples were 5.14+0.45 to 5.80+0.45 mg gallic acid equivalent/g, 4.68+0.34 to 5.81+1.39 mg ascorbic acid equivalent/g and 41.08+9.17 to 45.56+8.80 μmol FeSO4/g, respectively. There were no significant differences between the unirradiated sample and the irradiated samples. In conclusion, electron beam irradiation was an effective method for microbial decontamination of raw herbal material without affecting the total phenolic content and the antioxidant activity of C. asiatica.

King Mongkut's University of Technology North Bangkok. Central Library

Address: BANGKOK

Email: library@kmutnb.ac.th

Created: 2017

Modified: 2562-08-16

Issued: 2019-08-16

บทความ/Article

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BibliograpyCitation : In Chemical Society of Thailand under the Patronage of Her Royal Hghness Princess Chulabhorn Mahidol and King Mongkut's University of Technology North Bangkok Faculty of Applied Science. Pure and Applied Chemistry International Conference (PACCON 2017) (pp.1022-1026). Bangkok : Chemical Society of Thailand under the Patronage of Her Royal Hghness Princess Chulabhorn Mahidol

eng

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