Proteomics study of Saccharomyces cerevisiae grown under different culture temperatures

การศึกษาผลกระทบของอุณหภูมิที่ใช้ในการเจริญของยีสต์ Saccharomyces cerevisiae ในระดับโปรตีโอม

Name: Krittiya Kriengsaksri

Organization : King Mongkut's University of Technology Thonburi. School of Bioresources and Technology. Biotechnology

keyword: Proteome analysis

; Lipid metabolism

; การศึกษาในระดับโปรตีโอม

; ลิปิดเมตาโบลิสม

Abstract: The temperature has been known to have a significant effect on the lipid content in Saccharomyces cerevisiae. Researchers have attempted to understand the low temperature response mechanism at the transcriptomic and metabolomic levels.However, its mechanism is still unclear. In this work, we have focused on proteomics approach to explain how low temperature affects lipid metabolism, by using S.cerevisiae as a model. Yeast proteome literatures mining revealed that most of proteins detected by the two-dimensional gel electrophoresis (2D-PAGE) are soluble proteins.Thus we first developed the proper reagents for extracting both soluble and membranebound proteins for the yeast system. Each protein portion was separated by 2D-PAGE with proper sets of conditions. Then, the data was analyzed by pI and molecular weightmatching as well as the spot pattern matching. The low temperature was found to induce the protein involving in the transportation and amino acid metabolism. More importantly, the low temperature induced the soluble proteins hnctioning in the metabolism of some lipids including ergosterol, sphingolipid and phospholipids.Moreover, the membrane proteins in the "cell structure and cell polarity" category were induced by the low temperature. Furthermore, the low temperature reduced the soluble .proteins, particularly the enzymes in the "protein folding" category and the membrane proteins in the "transcription" category. Interestingly, the results of the spot pattern matching showed that the fructose-biphosphate aldolase 1 (FBAl) was down-regulated at the low temperature condition. However, the soluble proteins in the "glycolysis" except FBAl and the membrane proteins in the "translation" were not affected by the low growth temperature. This study provides preliminary information of how low temperature affects the lipid and other metabolisms of S. cerevisiae at protein level.

King Mongkut's University Technology Thonburi. Library

Address: กรุงเทพมหานคร (Bangkok)

Email: info.lib@mail.kmutt.ac.th

Name: Asawin Meechai

Role: Advisor

Name: Supapon Cheevadhanarak

Role: Advisor

Created: 2004

Issued: 2007-07-17

Modified: 2007-07-26

วิทยานิพนธ์/Thesis

ISBN: 9741841078

CallNumber: BIT508

eng

DegreeName: Master of Science

Level: Master's Degree

Descipline: Biotechnology

Grantor: King Mongkut's University of Technology Thonburi

©copyrights King Mongkut's University of Technology Thonburi

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