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Nathawut Choengchan. Method development for 'point-of-care' analysis of uric acid in urine using an 'in-house' LED spectrometer. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2017.
Method development for 'point-of-care' analysis of uric acid in urine using an 'in-house' LED spectrometer
Organization :
King Mongkuts Institute of Technology Ladkrabang. Faculty of Science
Email :
a.mathawee@live.com
Organization :
King Mongkuts Institute of Technology Ladkrabang. Faculty of Science
Name: Treerat Troangjaroensub
Organization :
King Mongkuts Institute of Technology Ladkrabang. Faculty of Science
Name: Napasporn Chatapattama
Organization :
King Mongkuts Institute of Technology Ladkrabang. Faculty of Science
Name: Noppadol Maneerat
Organization :
King Mongkuts Institute of Technology Ladkrabang. Faculty of Engineering
Name: Nathawut Choengchan
Organization :
King Mongkuts Institute of Technology Ladkrabang. Faculty of Science
Email :
nchoengchan@gmail.com
keyword:
In-house
LCSH:
Point-of-care systems
LCSH:
Uric acid
LCSH:
Light emitting diodes
LCSH:
Spectrometer
Abstract:
In this work, the method for 'point-of-care' analysis of uric acid in urine using the
'in-house' light emitting diode (LED) spectrometer was presented. Detection chemistry was
based on the colorimetric reaction between uric acid and phosphotungstic acid in the presence
of sodium carbonate. Blue-colored product was developed and its corresponded absorbance
was monitored by the LED spectrometer. Analytical performances of the method using the
LED spectrometer were investigated. Working range from 4.46 to 62.44 mg dL-1 was observed
with good linearity (R2 > 0.99). The method also provided good precision (RSD: 2.9 to 5.7 %)
and accuracy (Analytical recovery: 88 to 95 %). The limit of detection (yB+3SB) and the limit
of quantitation (yB+10SB) were found at 2.33 and 7.38 mg dL-1, respectively. The method was
applied to spiked urine samples collected from normal volunteers. The results obtained by the
LED spectrometer and by the UV-Vis spectrophotometer were not significant difference by
paired t-test at 95 % confidence (tstat. = 1.44, tcri. = 2.26). These results implied that the method
based on using LED spectrometer suitable and high precision for the quantitative point-ofcare
analysis of uric acid in urine sample.
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2017
Modified:
2562-07-23
Issued:
2019-07-23
บทความ/Article
application/pdf
BibliograpyCitation :
In Chemical Society of Thailand under the Patronage of Her Royal Hghness Princess Chulabhorn Mahidol and King Mongkut's University of Technology North Bangkok Faculty of Applied Science. Pure and Applied Chemistry International Conference (PACCON 2017) (pp.156-161). Bangkok : Chemical Society of Thailand under the Patronage of Her Royal Hghness Princess Chulabhorn Mahidol
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
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|---|---|---|
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