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Fuangfa Unob. Development of urinary albumin and creatinine detection kit. (). King Mongkut's University of Technology North Bangkok. Central Library. : , 2017.
Development of urinary albumin and creatinine detection kit
Name: Lalitphan Hongtanee
Organization :
Chulalongkorn University. Faculty of Science
Email :
Lalitphan.h@gmail.com
Name: Luxsana Dubas
Organization :
Chulalongkorn University. Faculty of Science
Name: Fuangfa Unob
Organization :
Chulalongkorn University. Faculty of Science
keyword:
Urinary albumin
LCSH:
Creatinine
LCSH:
Polyelectrolytes
-- Analysis
Abstract:
Albumin-to-creatinine ratio is the important diagnostic marker for kidney disease.
The colorimetric determination of urinary albumin and creatinine was developed in this
research. The cellulose acetate membrane (CA) was modified with polyelectrolyte multilayers
(PEMs) using the layer-by-layer deposition technique (LbL). Poly(diallyl dimethyl
ammonium chloride (PDADMAC) and poly(sodium 4-styrenesulfonate) (PSS) were selected
as a suitable polyelectrolyte pair. PEMs film growth on the membrane was characterized
using UV-Vis and ATR-FTIR. The modified membrane which PDADMAC is the outermost
layer was used to filter bovine serum albumin (BSA) solutions using a syringe pump and the
other membrane which PSS is the outermost layer was dipped into creatinine solution.
Albumin and creatinine were absorbed onto the modified membranes via electrostatic
interaction. Tetrabromophenol blue (TBPB) in Triton X-100 solution was used to analyze
albumin by forming blue ion complexes with a max of 625 nm, while Jaffe reaction was used
to detect creatinine. The factors influencing the albumin detection including the concentration
of TBPB, Triton X-100 and pH of desorption solution have been investigated. The results
showed that the optimized albumin detection is achieved using 0.03 mM TBPB in 0.2% (w/v)
Triton X-100 and citric-citrate buffer pH 3 as desorption solvent. The color intensity of
creatinine complexes provided a linear range for creatinine detection in the range of 0 100
g/L. The modified membranes and analytical method would be useful as a simple and costeffective
detection method for the early diagnosis of kidney failure.
King Mongkut's University of Technology North Bangkok. Central Library
Address:
BANGKOK
Email:
library@kmutnb.ac.th
Created:
2017
Modified:
2562-07-23
Issued:
2019-07-23
บทความ/Article
application/pdf
BibliograpyCitation :
In Chemical Society of Thailand under the Patronage of Her Royal Hghness Princess Chulabhorn Mahidol and King Mongkut's University of Technology North Bangkok Faculty of Applied Science. Pure and Applied Chemistry International Conference (PACCON 2017) (pp.102-107). Bangkok : Chemical Society of Thailand under the Patronage of Her Royal Hghness Princess Chulabhorn Mahidol
eng
©copyrights King Mongkut's University of Technology North Bangkok
RightsAccess:
ใช้เวลา
0.021711 วินาที
Lalitphan Hongtanee
| Title | Contributor | Type |
|---|---|---|
| Development of urinary albumin and creatinine detection kit
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Lalitphan Hongtanee;Luxsana Dubas;Fuangfa Unob | บทความ/Article | |
| Development of urinary albumin detection kit
จุฬาลงกรณ์มหาวิทยาลัย Lalitphan Hongtanee | Luxsana Dubas Fuangfa Unob | วิทยานิพนธ์/Thesis |
| Colorimetric detection of norfloxacin and ceftazidime by modified materials
จุฬาลงกรณ์มหาวิทยาลัย Lalitphan Hongtanee | Fuangfa Unob Narong Praphairaksit | วิทยานิพนธ์/Thesis |
Luxsana Dubas
| Title | Contributor | Type |
|---|---|---|
| Development of urinary albumin and creatinine detection kit
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Lalitphan Hongtanee;Luxsana Dubas;Fuangfa Unob | บทความ/Article |
Fuangfa Unob
| Title | Contributor | Type |
|---|---|---|
| Colorimetric detection of cysteine using silver-hydroxyapatite nanoparticles
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Retno Prasetia;Fuangfa Unob | บทความ/Article | |
| Development of urinary albumin and creatinine detection kit
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Lalitphan Hongtanee;Luxsana Dubas;Fuangfa Unob | บทความ/Article | |
| Consecutive removal of basic dye and reactive dye using biological sludge from wastewater treatment process
มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าพระนครเหนือ Jirakit Paiyarat;Fuangfa Unob | บทความ/Article |