แบบจำลองทางคณิตศาสตร์ของวงจรการแบ่งเซลล์ของยีสต์ขนมปัง

Name: สุวัฒณี จงจิตตาภิบาล

Name: Suwattanee Chongchittapiban

keyword: baker's yeast

; cell cycling model

; growth model

; Saccharomyces cerevisiae

Abstract: Batch and fed-batch cultures of baker's yeast were studied experimentally.Effect of initial glucose concentration on the growth of baker's yeast cells was observed in batch experiments by varying initial glucose concentrations at 5, 10, 20 and 40 g/l. A mathematical model was developed in order to describe the dynamic changes of glucose, biomass and ethanol concentrations in batch culture. This growth kinetic model was proposed based on diauxic growth characteristic of yeast cells. As a set of ordinary differential equations, the model was solved numerically. The parameters of the model were identified using the experimental data. The simulation results fitted well with the experimental data with correlation coefficient (R~) > 0.9. Values of the model's parameters obtained fiom experiments conducted using initial glucose concentrations of 5, 10 and 20 g/l are same whereas 40 g/l initial glucoseresult was slightly different.A simple cell cycling model was developed to predict the cell populationdynamic of baker's yeast fermentation in batch culture. The cell cycling model was combined with the developed growth kinetic and solved numerically together with the growth kinetic model. Values of parameters of the cell cycling model were estimated by fitting the modeling results with the results of the cell counts in batch experiments. The values of the model's parameters were found to be similar for all batch experiments conducted in this work. The model showed a good fit with the experimental data, therefore, it can be used to predict the value of fraction of budding cell (FBC) in batch fermentation of baker's yeast using glucose as substrate. The combination of the growth kineticmodel and cell cycling model was further developed for fed-batch fermentation of baker's yeast. The parameters of the new model were identified and found that they deviated fiom those of batch fermentation. Since only one set of the values of the model's parameters could not be used in the model predictions of the cell population concentrations throughout the culture, the values of the model's parameters of fed-batch fermentation might be changed along with the culture period

มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

Address: กรุงเทพมหานคร (Bangkok)

Email: info.lib@mail.kmutt.ac.th

Name: Dr. Anan Tongta

Role: Supervisor

Created: 2542

Issued: 2006-01-11

Modified: 2553-07-10

วิทยานิพนธ์/Thesis

ISBN: 9746247891

CallNumber: BIT293

tha

DegreeName: วิทยาศาสตรมหาบัณฑิต

Level: ปริญญาโท

Descipline: เทคโนโลยีชีวภาพ

Grantor: มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

©copyrights มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

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3	BIT293ab.txt	2.44 KB	21	2018-05-13 06:45:47

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