การศึกษารูปแบบของไซลาโนไลติกโปรตีนจากจุลินทรีย์ในกลุ่ม mesophilic

Study on xylanolytic protein patterns of mesophilic microorganisms

Name: จิรภา ทองสุข

Organization : มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

keyword: xylanolytic protein patterns

; SDS-PAGE

; active-PAGE

; multiple xylanases

; mesophilic xylanase

Abstract: The study on the effect of carbon sources on xylanolytic protein patterns showed thatxylanolytic protein patterns could be changed, depending on carbon sources. Bacillus circulansB6, cultured with xylan as the best carbon source, produced more xylanolytic proteins (morethan 16 isozymes), when compared with other carbon sources. That could be proved by thexylanolytic proteins produced from Bacillus spp. as the standard strains such as B. subtilis (fromsoy bean fermented), B. subtilis (from waste water of cassava factory), B megaterium, B.licheniformis, B. circulans Bd, Bacillus sp. H2 and Bacillus sp. S1 under the same condition ofcultivation. It was found that the xylanolytic protein patterns on SDS-PAGE and active-PAGE ofeach species were different. Therefore, this method can be used for identification of the differentsub-species of Bacillus.The six Bacillus strains which isolated from a waste water treatment plant of pulpand paper industry at Ban-pa-in, Ayutaya was cultivated at pH 10. The physiological andbiological properties of these bacteria showed the same results. However, xylanolytic proteinpatterns from SDS-PAGE and active-PAGE showed that the six strains could be classified intotwo groups. The first group was AX-2 and AX-13 produced the 45 kDa xylanase. The second ,group, AX-4, AX-8, AX-10 and AX-12 produced the 16 and 45 kDa xylanases. In addition,when they were cultivated at pH 7.0, it showed that they could be classified into four groups.The first group AX-2 produced the 21 and 45 kDa xylanases. The second, AX-4, AX-8 and AX-10 produced the 45 kDa xylanases. The third, AX-12 produced the 45 kDa xylanase which wassimilar to the second group, but the xylanolytic protein patterns on SDS-PAOE are different. Thelast group, AX-13 produced the 21,45 and 51 kDa xylanases. Thus, initial pH of media appearedto affect on the production of xylanolytic proteins and used to c o n f i the differentmicroorganisms by this technique.The study on xylanolytic protein patterns of all microorganisms tested showed thatmost of microorganisms produced multiple forms of xylanases. Alternatively, allmicroorganisms produced xylanases as major bands which could be classified into two groups.First at pH 7, Bacillus spp. and microorganisms isolated from 1. a waste water treatment plantof pulp and paper industry at Bang-pa-in, Ayutaya 2. from Siam pulp and paper at Ratchaburiand 3. from compose fertilizer produced the 25,45,32 and 29 kDa xylanases, respectively as t bmajor proteins. Second, at pH 10, all microorganisms tested had the 45 kDa xylanase as themajor protein. Ftutherrnore, the xylanases produced from all BacilIus spp. could be classifiedinto two groups depended on the size such as the low molecular weight (16 to 25 kDa) and thehigh molecular weight (43 to 50 kDa) xylanases.

มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

Address: กรุงเทพมหานคร (Bangkok)

Email: info.lib@mail.kmutt.ac.th

Name: รศ.ดร.กนก รัตนกนกชัย

Role: อาจารย์ที่ปรึกษา

Created: 2543

Issued: 2005-10-28

Modified: 2006-05-30

วิทยานิพนธ์/Thesis

ISBN: 9746281801

CallNumber: BIT326

tha

DegreeName: วิทยาศาสตรมหาบัณฑิต

Level: ปริญญาโท

Descipline: เทคโนโลยีชีวภาพ

Grantor: มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

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