การเหนี่ยวนำ xylanolytic enzymes ใน alkaliphilic Bacillus firmus K-1

Induction of xylanolytic enzymes in alkaliphilic Bacillus firmus K-1

Name: ปิยนุสร์ น้อยด้วง

Organization : มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

keyword: Bacillus Jirmus K-11 xylanolytic enzymes

; constitutive enzyme

; inducible enzyme

; xylan-binding xylanase

Abstract: The induction of xylanolytic enzymes in the alkaliphilic bacterium, Bacillusfzrnzus K-1,by various carbon sources such as oat spelt xylan, hctose, glucose, arabinose and xylose (0.5%W/V) was investigated. Oat spelt xylan was found to be the best inducer for production ofxylanolytic enzymes. Arabinofuranosidase was induced by xylan and arabinose and Pxylosidasewas induced by xylan and xylose. SDS-PAGE showed that they had the molecularmasses of 35 and 52 kDa, respectively. All carbon soutces tested induced xylanase and acetylesterase production. Acetyl esterase found to be constitutive. The result fr.om active-PAGEindicated that Bacillusjhus K-1 produced two xylanases that had molecular masses of 23 and45 kDa The 23 kDa xylanase was a xylan-binding xylanase which had the ability to bind toinsoluble xylan due to the presence of xylan-binding region. The 45 kDa xylanase was unboundxylanase, which was produced by all carbon sources except glucose, indicating that 45 kDaxylanase was repressed by glucose but no effect on the 23 kDa xylanase production Therefore,the effect of various concentrations (0.1-1.M) of glucose on xylanase production was conductedand it was found that various ammbaticms of gl- repressed the produdion of 45 kDaxylanase whereas 23 kDa xylanase production i n d with increasing of glucoseconcentrations but high concentrations of glucose delayed the xylanase proddon. Productionof 23 kDa xylanase did not occur until glucose -on in culture medium was less than 1g/I. M m e r , time course of xylanase production by using oat spelt xylan as a carbon sourcewas also studied and it was found that 23 kDa xylanase was produced before the production of45 kDa xylanase and other debranching enzymes.

มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

Address: กรุงเทพมหานคร (Bangkok)

Email: info.lib@mail.kmutt.ac.th

Name: ดร.คิน เลย์ คู

Role: อาจารย์ที่ปรึกษา

Created: 2543

Issued: 2005-10-28

Modified: 2006-05-30

วิทยานิพนธ์/Thesis

ISBN: 9746283839

CallNumber: BCT27

tha

DegreeName: วิทยาศาสตรมหาบัณฑิต

Level: ปริญญาโท

Descipline: เทคโนโลยีชีวเคมี

Grantor: มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

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