การสังเคราะห์และศึกษาคุณสมบัติทางเคมีไฟฟ้าของพอลิเมอร์เมมเบรนของ redox active biocompatible membrane สำหรับงานด้านไบโอเซนเซอร์ทางคลีนิคการแพทย์

Synthesis and Electrochemical Characterization of Redox-ActiveBiocompatible Polymer Membranes for Use in Clinical Biosensor

Name: จตุพร พานทอง

Organization : มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

keyword: Biocompatibility

; Biosensor

; Redox-active polymer membrane

; Poly(viny1alcohol)

; Poly(ethy1ene glycol)

; Ferrocene.

Abstract: The research involved synthesis and characterization of redox-active biocompatiblepolymer membranes base on poly(viny1 alcohol)-poly(ethy1ene glycol) [PEG-PVA] andpoly(viny1 alcohol)-ferrocene [PVA-Fc] for use in clinical biosensors. PEG-PVA was madeby generating an aldehyde group on PEG and then using this to bind PVA via cyclic acetalformation. The IR spectrum of PEG-PVA compared to that of PEG-CHO, showed thecyclic acetal peaks at 1,205, 1,187, 1,124 and 1,099 cm-1. The BSA resistance of PEGPVA-modified glassy carbon electrode was considered by studying characteristic of cyclicvoltammogram of 1.0 K3Fe(CN)6 in 5 gl-1 BSA solution and compared the current withPVA modified GC. PEG-PVA and PVA modified electrode were not adsorbed by BSAand currents were stable at 97.9 % and 91.2% respectively.The PVA-Fc was synthesized by two different methods and compared electrochemicalsignals. Firstly, The -OH along the PVA backbone was functionalised to incorporateferrocene methanol through the linking agent cyanuric chloride. Secondary, OH-groupsalong the PVA backbone formed cyclic acetal with ferrocene carboxaldehyde. The PVA-Fcgels were coated on a glassy carbon electrodes. The current of PVA bound ferrocenemethanol lost completely after 20 minutes but the current of PVA bound ferrocenecarboxaldehyde was stable at 0.71 pA. However, the Fc loading was relatively low(molecular ratio 1:2 x 10 4). Voltammetry of the modified electrode revealed stablepotential eaks for the redox couple at 0.3 V and 0.2 V vs SCE. The surface coverage was 3.1 x 10-9 mol c m -2 . The redox couple was found to be quasi-reversible with anodic andcathodic transfer coefficients of 0.5 and 0.7 res ectively. The the oxidation and reduction P rate constants were found to be 20 s-1 and 30 s- respectively at a scan rate 1.0 VS-1. PVAFc also showed resistance to BSA adsorption.Redox active biocompatible membrane, PEG-PVA-Fc was synthesized by forming cyclicacetal between PEG-CHO and PVA-Fc and characterized both of electrochemical propertyand protein adsorption. Electrochemical study of PEG-PVA-Fc was unsuccessful. Since thePEG-PVA-Fc obtained was electrochemically inactive.

มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

Address: กรุงเทพมหานคร (Bangkok)

Email: info.lib@mail.kmutt.ac.th

Name: Asst.Prof.Dr. Werasak Surareungchai

Role: Supervisor

Created: 2545

Issued: 2005-10-28

Modified: 2006-05-10

วิทยานิพนธ์/Thesis

ISBN: 974955146X

CallNumber: BIT412

tha

DegreeName: วิทยาศาสตรมหาบัณฑิต

Level: ปริญญาโท

Descipline: เทคโนโลยีชีวภาพ

Grantor: มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

©copyrights มหาวิทยาลัยเทคโนโลยีพระจอมเกล้าธนบุรี

ลำดับที่.	ชื่อแฟ้มข้อมูล	ขนาดแฟ้มข้อมูล	จำนวนเข้าถึง	วัน-เวลาเข้าถึงล่าสุด
1	BIT412.pdf	1.92 MB	96	2023-12-27 11:16:47
2	BIT412ab.pdf	118.62 KB	44	2018-05-13 04:55:34
3	BIT412ab.txt	2.6 KB	29	2018-05-13 04:55:35

ใช้เวลา

0.019054 วินาที